驻岛士兵皮肤屏障功能与皮肤癣菌感染的相关性研究

发布时间：2018-01-03 13:01

本文关键词：驻岛士兵皮肤屏障功能与皮肤癣菌感染的相关性研究　出处：《桂林医学院》2013年硕士论文　论文类型：学位论文

【摘要】：目的：研究驻岛士兵皮肤屏障功能与皮肤癣菌感染的相关性。方法：1.皮肤屏障功能检测，，反映皮肤屏障功能的三个指标（经表皮丢失水量、皮脂含量、角质层含水量）均使用德国CK公司生产的多功能皮肤测试仪MPA9进行测量；测试环境温度为22~24℃，相对湿度为46%~55%；检测皮肤屏障功能前，研究对象先进入指定地方适应环境15分钟等待检测。受测部位为面部、腹股沟区，在受试部位每个指标进行3次测量，取平均值。角质层含水量和皮脂含量的单位均为au (arbitraryunit)，经表皮水分丢失量的单位为g/m2h。2.真菌检查，符合条件的93名研究对象均在相应部位无菌条件下刮取皮屑进行真菌镜检和培养。采用15%KOH溶液进行镜检，采用沙式琼脂培养基(含0.25%放线菌酮的葡萄糖、氯霉素、蛋白胨、琼脂培养基),平皿多点接种或双试管进行培养,25~27℃培养。结果：面部各组的经表皮水分丢失量均值之间比较无明显统计学意义（F=0.611，P＞0.05）；即面部所测样本中经表皮水分丢失量各组无明显差异，与皮肤癣菌感染无相关;腹股沟各组的经表皮水分丢失量均值之间比较可见明显统计学意义（F=104.525，P＜0.05），两两比较的结果都是P＜0.05，即真菌阳性组与真菌阴性组、真菌阳性组与正常对照组、真菌阴性组与正常对照组之间比较具有明显统计学差异，即腹股沟所测样本中经表皮水分丢失量各组有明显差异，与皮肤癣菌感染有相关性。面部各组的皮脂含量均值之间比较有明显统计学意义（F=95.461， P＜0.05），两两比较亦均P＜0.05，即真菌阳性组与真菌阴性组、真菌阳性组与正常对照组、真菌阴性组与正常对照组之间比较具有明显统计学差异，即所测样本面部皮脂含量与皮肤癣菌感染有相关性；腹股沟各组的皮脂含量均值之间比较无明显统计学意义（F=1.29， P＞0.05），即所测数据腹股沟皮脂含量与皮肤癣菌感染无相关性。面部各组的角质层含水量均值之间比较无明显统计学意义（F=0.840，P＞0.05），即所测数据腹股沟角质层含水量与皮肤癣菌感染无相关性。腹股沟各组的角质层含水量均值之间比较无明显统计学意义（F=3.015，P＞0.05），即所测数据腹股沟角质层含水量与皮肤癣菌感染无相关性。面部真菌阳性组、真菌阴性组、正常对照组所测样本中经表皮水分丢失量、皮脂含量、角质层含水量与年龄、军龄、驻岛时间之间关系均无明显统计学意义（P＞0.05）。腹股沟真菌阳性组所测样本中经表皮水分丢失量、皮脂含量、角质层含水量与年龄、军龄、驻岛时间之间关系均无明显统计学意义（P＞0.05）；腹股沟真菌阴性组与正常对照组所测样本中经表皮水分丢失量、角质层含水量与年龄、军龄、驻岛时间之间关系无明显统计学意义（P＞0.05）；由于腹股沟真菌阴性组与正常对照组所测样本中皮脂含量均为0，无法计算出其与其他因素的关系。结论：（1）驻岛部队士兵患皮肤癣菌感染的腹股沟经表皮水分丢失量与皮肤癣菌感染存在相关性；（2）驻岛部队士兵患皮肤癣菌感染的面部皮脂含量亦与皮肤癣菌感染存在相关性。（3）驻岛部队士兵患皮肤癣菌感染的面部、腹股沟角质层含水量与皮肤癣菌感染无相关性。（4）驻岛部队士兵患皮肤癣菌感染的面部、腹股沟皮肤屏障功能与年龄、军龄以及驻岛时间无相关性。
[Abstract]:Objective: To study the relationship between the skin barrier function of the soldiers in the island and the infection of dermatitis.
Methods: 1. skin barrier function test, three indexes of skin barrier function (transepidermal water loss, sebum content, water content in stratum corneum) were using the MPA9 multifunctional skin test instrument produced by German CK were measured; the test environment temperature of 22~24, the relative humidity is 46%~55%; detection of skin barrier function. The research object first enter the designated place to adapt to the environment for 15 minutes waiting for detection. The measured parts for facial, inguinal area in the subjects part of each indicator for measuring 3 times, the average. The cuticle moisture and sebum content units are Au (arbitraryunit), transepidermal water loss per unit of g/m2h.2. fungi for inspection, 93 subjects were eligible in the corresponding position under aseptic conditions to scrape scurfy microscopy and fungal culture. Microscopic examination using 15%KOH solution, using sand type agar medium (containing 0.25% line The glucose of ketone, chloramphenicol, peptone, agar medium, multi point inoculation or double test tube were cultured and cultured at 25~27.
Results: the face of each transepidermal water loss between the average value was not statistically significant (F=0.611, P > 0.05); facial samples in transepidermal water loss was not obvious difference, and dermatophyte infection related; transepidermal water loss is obvious statistical significance between mean inguinal groups (F=104.525, P < 0.05), the 22 results are P < 0.05, the fungal positive group and negative group of fungi, fungal positive group and normal control group, compared with significant statistical difference between fungal negative group and normal control group, the groin samples in transepidermal water loss groups have obvious difference that is associated with dermatophyte infection.
Comparison was statistically significant between the mean of each facial sebum content (F=95.461, P < 0.05), 22 were P < 0.05, the fungal positive group and negative group of fungi, fungal positive group and normal control group, compared with significant statistical difference between fungal negative group and normal control group, which is measured by the facial sebum the content of sample and dermatophyte infection have correlation between mean sebum content; inguinal groups was not statistically significant (F=1.29, P > 0.05), the test had no correlation with inguinal sebum content data of dermatophyte infection.
The cuticle facial moisture mean between groups was not statistically significant (F=0.840, P > 0.05), i.e. the data measured by the water content of stratum corneum and inguinal dermatophyte infection. No correlation between cuticle water content between the mean inguinal groups had no significant statistical significance (F=3.015, P > 0.05), i.e. the measured data of inguinal water content in stratum corneum and dermatophyte infection had no correlation.
The face of fungal fungal positive group and negative group, normal control group samples in transepidermal water loss, sebum content, water content in stratum corneum with age, length of military service, the relationship between the island time were no statistical significance (P > 0.05).
Inguinal fungal samples positive group in transepidermal water loss, sebum content, water content in stratum corneum with age, length of military service, the relationship between the island time were no statistical significance (P > 0.05); inguinal fungal negative group and normal control group samples in transepidermal water loss, water content in stratum corneum with age, the relationship between the time of military service, stationed in the island was not statistically significant (P > 0.05); because of a groin fungal negative group and normal control group by measuring the sebum content in samples was 0, to calculate its relationship with other factors.
Conclusions: (1) there is a correlation between the loss of the epidermis of the groin and the infection of the derma of the troop infected soldiers in the island.
(2) the content of the skin sebum in the soldiers suffering from dermworm infection in the island troops is also associated with dermatitis infection.
(3) there was no correlation between the water content of the horny layer of the groin and the infection of dermworm infection in the soldiers suffering from dermatitis in the island troops.
(4) the garrison soldiers suffering from dermatophyte infection face, groin skin barrier function and age, no correlation between military service and stationed in the island.

【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R82

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