高原兔肝脏火器伤后机体应激性变化的研究

发布时间：2018-01-17 23:15

本文关键词：高原兔肝脏火器伤后机体应激性变化的研究　出处：《石河子大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的：探讨兔在高原环境下肝脏火器伤后血清皮质醇(Cor)、促肾上腺皮质激素(ACTH)、丙二醛(MDA)、超氧化物歧化酶(SOD)、全血谷胱甘肽(GSH)及肺组织糖皮质激素受体(GR)的动态变化规律及其意义,旨在提高高原肝外伤的综合救治水平,降低肝外伤的死亡率,同时也为防治高原肝脏火器伤后机体多器官功能障碍综合症提供理论依据。方法:采用成年健康新西兰大白兔144只,随机分为平原肝脏火器伤组(A组)、初入高原肝脏火器伤组(B组)、适应高原肝脏火器伤组(C组),各组又细分为正常对照组和伤后0.5、2、6、12、24、48、72h,每组6只。在不同海拔环境下成功建立肝脏火器伤模型后,分别于各时间点采集血液标本和左下肺组织,采用固相夹心法酶联免疫吸附实验测定各组血清Cor和ACTH;硫代巴比妥酸法测定各组血清MDA含量；黄嘌呤氧化酶法测定各组血清SOD活力；比色定量法测定各组全血GSH含量；免疫组织化学图像分析法测定各组肺组织GR的表达。结果:①与正常组比较,肝脏火器伤后3组兔血清Cor、ACTH含量持续升高,6h达峰值(P0.01),12h仍处于较高水平,之后进行性下降；伤后MDA含量持续升高,12h达峰值(P0.01),继而进行性下降,72h仍高于正常,而SOD活力、GSH含量早期略有升高,以后逐渐下降,到达最低值后逐渐恢复；伤后GR表达开始下降,最低点出现在6h,之后缓慢恢复,72h仍未恢复至正常水平。②与平原火器伤组比较,初入高原正常组Cor、ACTH含量已高于平原正常组(P0.01),致伤后升高幅度及最高值水平也更高；MDA到达峰值水平(P0.01)和SOD活力、GSH含量早期升高及以后到达最低值水平更显著；GR表达致伤后下降幅度及最低值水平也更显著(P0.01)。③与平原火器伤组比较,适应高原各组Cor、ACTH含量升高幅度及到达最高值水平；MDA升高幅度和SOD活力、GSH含量降低幅度(P0.05);GR表达下降幅度及最低值水平都更显著,但其显著程度又低于初入高原组。结论:①高原肝脏火器伤后,在创伤和高原低氧双重应激的影响下,机体产生了明显的应激反应,其程度明显强于平原创伤应激；②火器伤打破了体内氧化与抗氧化之间的平衡,同时,高原低氧可使机体产生过多的自由基,并且使机体清除自由基的能力下降,氧自由基可能参与火器伤后机体的病理生理过程；③GR表达下调可引起其功能受抑制,特别是抗炎作用减弱,从而导致全身炎症反应综合症,可能是多器官功能衰竭的重要原因。
[Abstract]:Objective: to investigate the levels of serum cortisol cortisone, adrenocorticotropic hormone (ACTH), malondialdehyde (MDA) and superoxide dismutase (SOD) in rabbits after hepatic firearm injury at high altitude. The dynamic changes of glutathione glutathione (GSH) and glucocorticoid receptor (GRG) in lung tissue were studied in order to improve the comprehensive treatment level of liver trauma at high altitude and reduce the mortality of liver injury. It also provides theoretical basis for prevention and treatment of multiple organ dysfunction syndrome after firearm injury of liver at high altitude. Methods: 144 adult healthy New Zealand rabbits were randomly divided into two groups: group A with hepatic firearm injury in plain area, group B with firearm injury group with liver firearm injury at high altitude, group C with firearm injury group adapted to high altitude. Each group was subdivided into two groups: normal control group and normal control group, and 6 rats in each group for 72 h. The model of hepatic firearm injury was successfully established at different altitude. Blood samples and left lower lung tissues were collected at each time point, and serum Cor and ACTH were measured by solid phase sandwich enzyme-linked immunosorbent assay (Elisa). The content of serum MDA was determined by thiobarbituric acid method. The activity of serum SOD was determined by xanthine oxidase method. The content of GSH in whole blood of each group was determined by colorimetric quantitative method. The expression of gr was detected by immunohistochemical image analysis. Results compared with the normal group, the serum level of Cora ACTH in the three groups was still at a higher level at 6 h after firearm injury than that in the normal group at 12 h, and then decreased gradually. The content of MDA increased continuously at 12h, reached the peak value (P0.01A), then decreased gradually. The content of SOD increased slightly at the early stage of injury, and then decreased gradually. After reaching the lowest value, it gradually recovered; After injury, the expression of gr began to decrease, the lowest point appeared at 6 hours, and the slow recovery after 72 hours did not return to the normal level of 2.2.Compared with the firearm injury group in plain, the Cor in the normal group first entered the plateau. The content of ACTH was higher than that of the plain normal group (P 0.01), and the range of increase and the highest level after injury were higher than those in the normal group. MDA reached the peak level (P0.01) and SOD activity increased at the early stage and then reached the lowest level. The decrease and the lowest level of gr expression after injury were also more significant than those of plain firearm injury group, and the increase of Corn ACTH content and reaching the highest level in all groups at high altitude were better than those in plain firearm injury group. The increase of MDA and the decrease of GSH content in SOD activity were P0.05A; The decrease of gr expression and the lowest level of gr expression were more significant, but the significance of gr expression was lower than that of the new plateau group. Conclusion under the influence of trauma and high altitude hypoxic double stress, there was obvious stress response after firearm injury at high altitude, which was stronger than that in plain. (2) firearm injury broke the balance between oxidation and antioxidation in vivo. At the same time, hypoxia at high altitude could make the body produce excessive free radicals and decrease the ability of scavenging free radicals. Oxygen free radicals may be involved in the pathophysiological process of the body after firearm injury. The down-regulation of 3gr expression may result in the inhibition of its function, especially the weakening of its anti-inflammatory effect, which may lead to systemic inflammatory response syndrome, which may be an important cause of multiple organ failure.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R826.5

【参考文献】