兔坐骨神经火器伤后腰段脊髓脂质过氧化反应及意义

发布时间：2018-03-17 10:05

本文选题：火器伤　切入点：凋亡　出处：《中国急救医学》2002年01期 　论文类型：期刊论文

【摘要】：目的　探讨脂质过氧化反应在兔坐骨神经火器伤后腰段脊髓细胞凋亡中的意义。方法　火器伤组靶点为兔右后肢外侧坐骨神经体表投影线中点 ;切割伤组在同一致伤水平切断坐骨神经。用DNA电泳、TUNEL染色技术对腰段脊髓细胞进行凋亡定性检测 ,用流式细胞术进行定量检测。同时测腰段脊髓超氧化物歧化酶 (SOD)活性和丙二醛 (MDA)含量。结果　火器伤组 1周和 2周时 ,腰段脊髓神经元和胶质细胞发生凋亡 ,切割伤组仅在 4周时有少量运动神经元发生凋亡。火器伤组 1d时 ,MDA含量显著下降 (P 0 0 1 ) ,3d、1周、2周时 ,MDA含量显著升高 (P 0 0 1 ) ,切割伤组MDA含量变化不明显。结论　火器伤组细胞凋亡发生时间早、数量多 ,脂质过氧化反应是其发生的原因之一
[Abstract]:Objective to investigate the significance of lipid peroxidation in the apoptosis of lumbar spinal cord cells after sciatic nerve firearm injury in rabbits. Methods the target of firearm injury group was the midpoint of the projection line of the lateral sciatic nerve in the right hind limb. The sciatic nerve was transected at the same injury level in the incised group. The apoptosis of lumbar spinal cord cells was detected qualitatively by DNA electrophoresis and Tunel staining. The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in lumbar spinal cord were measured by flow cytometry. Results the neurons and glial cells of lumbar spinal cord were apoptotic at 1 and 2 weeks after firearm injury. In the incised group, only a few motor neurons apoptosis occurred at the 4th week, and the MDA content in the firearm injury group decreased significantly on the 1st day (P 0 01), and the MDA content increased significantly at 1 week and 2 weeks after the firearm injury (P < 0 01). Conclusion the time of apoptosis in firearm injury group is early and the quantity of apoptosis is high. Lipid peroxidation is one of the reasons for the change of MDA content in cut injury group.
【作者单位】：第三军医大学附属大坪医院野战外科研究所第六研究室第三军医大学附属大坪医院野战外科研究所第六研究室第三军医大学附属大坪医院野战外科研究所第六研究室第三军医大学附属大坪医院野战外科研究所第六研究室第三军医大学附属大坪医院野战外科研究所第六研究室第三军医大
【基金】：国家“973”重点基础研究项目 (G19990 54 2 0 6 )
【分类号】：R826.5

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