几株硝化菌的筛选及其初步鉴定

发布时间：2018-03-21 00:44

本文选题：硝化菌　切入点：PCR　出处：《解放军预防医学杂志》2002年03期 　论文类型：期刊论文

【摘要】：目的 :筛选、分离能够用于治理氨氮污染和军事坑道生活污物、污水的硝化菌。方法 :通过富集培养从土壤中筛选的硝化菌 ,在进行形态学检验和硝化速率测定后 ,设计引物扩增硝化菌的特征性基因NorB ,随机选择一个PCR产物进行测序 ,并与Genbank上的序列比较同源性。结果 :筛选到 7株能够降解亚硝酸盐的细菌 ,经革兰氏染色后发现全部为阴性 ,其菌落形态以小、圆、淡黄色为主 ,细菌形态多为短杆状 ;各菌株的硝化速率在 4 .35～ 4 .80 (mg·L 1·d 1)之间。用PCR均能扩增出预期大小片段。对其中的某一个PCR产物直接测序 ,与Genbank中的序列比较 ,发现该基因与硝化菌N .hamburgensis的Norb基因同源性高达 99%。结论 :可以初步断定 ,我们所筛选到的菌为硝化菌
[Abstract]:Objective: to screen and isolate nitrifying bacteria which can be used to control ammonia nitrogen pollution, military tunnel sewage and sewage. Methods: after morphological examination and nitrification rate determination, the nitrifying bacteria selected from soil were enriched and cultured. Primers were designed to amplify the characteristic gene NorB of nitrifying bacteria, and a PCR product was randomly selected for sequencing. Results: seven strains of bacteria capable of degrading nitrite were screened. It was found that all of them were negative by Gram staining. The colony morphology was small, round and light yellow, and most of the bacteria were short rod shape. The nitrification rate of each strain ranged from 4.35 to 4.80 mg 路L 1 路d 1. The expected size fragments could be amplified by PCR. One of the PCR products was sequenced directly and compared with the sequence in Genbank. The homology of this gene with the Norb gene of nitrifying bacteria N. hamburgensis is as high as 99%. Conclusion: it can be preliminarily concluded that the bacteria we screened are nitrifying bacteria.
【作者单位】：军事医学科学院卫生学环境医学研究所军事医学科学院卫生学环境医学研究所军事医学科学院卫生学环境医学研究所军事医学科学院卫生学环境医学研究所军事医学科学院卫生学环境医学研究所军事医学科学院卫生学环境医学研究所军事医学科学院卫生学环境医学研究所
【分类号】：R82

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