Nω-硝基-L-精氨酸甲酯对失重大鼠肺动脉反应性的影响

发布时间：2018-03-21 01:48

本文选题：失重　切入点：模拟　出处：《中国危重病急救医学》2006年12期 　论文类型：期刊论文

【摘要】：目的探讨Nω-硝基-L-精氨酸甲酯(L-NAME)对失重大鼠肺动脉反应性的影响。方法将16只雄性Wistar大鼠按随机数字表法分为对照组和尾悬吊组,每组8只。利用离体血管环灌流技术测量大鼠肺动脉环对苯肾上腺素(PE)的收缩反应和对乙酰胆碱(Ach)的舒张反应,以及L-NAME预处理后动脉环对PE的收缩反应。同时用硝酸还原酶法测定肺动脉组织一氧化氮(NO)含量。结果尾悬吊14d后大鼠肺动脉环对1×10-5~1×10-7mol/L浓度PE的收缩反应均显著下降(P均0.01);对1×10-6~1×10-8mol/LAch的舒张反应均显著增强(P均0.05);一氧化氮合酶(NOS)抑制剂L-NAME预处理后动脉环对PE的收缩反应恢复,与对照组比较差异均无显著性。肺动脉组织NO含量增加,两组差异有显著性(P0.05)。结论模拟失重引起肺动脉血管内皮细胞释放NO增加可能是导致其收缩反应性下降的原因之一。
[Abstract]:Objective to investigate the effect of N nitro -L- arginine methyl ester (L-NAME) on the effects of weightlessness reactivity of pulmonary artery in rats. Methods 16 male Wistar rats were randomly divided into control group and tail suspension group, 8 rats in each group. The isolated vascular rings of pulmonary artery perfusion technique in rats rings to phenylephrine (PE) and the contractile response to acetylcholine (Ach) relaxation, and L-NAME after pretreatment of artery rings contraction to PE. At the same time, nitric oxide in pulmonary tissues was determined using nitrate reductase method (NO). The content of 14d in tail suspended rat pulmonary artery ring contraction to 1 * 10-5~1 * 10-7mol/L PE concentrations were significantly decreased (P < 0.01); relaxation response to 1 * 10-6~1 * 10-8mol/LAch were significantly enhanced (P < 0.05); nitric oxide synthase (NOS) inhibitor L-NAME pretreated artery ring contraction to PE recovery, there was no significant difference compared with the control group. The NO content in the pulmonary artery increased. There was a significant difference between the two groups (P0.05). Conclusion the increase of NO released from the pulmonary artery endothelial cells caused by simulated weightlessness is probably one of the reasons for the decrease of the contractile responsiveness.

【作者单位】：
【基金】：全军武器装备预研基金项目(51400020104JB001) 全军医学科研“十五”基金重点项目(04Z020)
【分类号】：R85

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