大鼠皮肤实验性枪弹伤后MMP-1和MMP-3的表达研究

发布时间：2018-03-27 22:41

本文选题：枪弹伤　切入点：损伤时间　出处：《第三军医大学学报》2008年13期

【摘要】：目的探讨枪弹伤后大鼠皮肤MMP-1和MMP-3随时间变化的表达情况,为枪弹伤后的损伤时间判断提供依据。方法建立大鼠对照组和实验组模型。对照组断颈处死后直接取材;实验组分别于枪弹伤后0、1、3、6、12、18、24、48、72h取材(n=3)。取材后用Northern blot法对MMP-1和MMP-3的mRNA进行半定量检测。结果MMP-1和MMP-3在枪弹伤后即刻其表达就有增加,以后表达继续升高,一直呈上升趋势(P0.01,P0.05);而阴性对照组几乎没有表达。结论枪弹伤后大鼠皮肤MMP-1和MMP-3表达增加的规律性,对枪弹伤后损伤时间的推断有一定的价值。
[Abstract]:Objective to investigate the expression of MMP-1 and MMP-3 in the skin of rats after gunshot wound and to provide evidence for judging the time of injury after gunshot wound. Methods the models of control group and experimental group were established. In the experimental group, the mRNA of MMP-1 and MMP-3 were detected by Northern blot method for 72h after gunshot wound. Results the expression of MMP-1 and MMP-3 increased immediately after gunshot wound, and then the expression continued to increase. Conclusion the increased expression of MMP-1 and MMP-3 in the skin of rats after gunshot injury has a certain value in the estimation of injury time after gunshot injury.
【作者单位】：重庆医科大学医学检验系重庆医科大学医学检验系重庆刑事科学技术研究所重庆医科大学医学检验系重庆刑事科学技术研究所
【分类号】：R826

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