适用于空间细胞培养系统的泵头分体式直流电机驱动微泵

发布时间：2018-03-28 00:27

  本文选题：微泵　切入点：直流电机驱动　出处：《航天医学与医学工程》2017年05期

【摘要】：目的为了搭建适用于我国航天医学细胞生物学研究的小型化、集成化、自动化细胞培养系统,提出了一种性能可靠、操作维护简单、成本低廉且自带阀控功能的泵头分体式直流电机驱动型蠕动微泵。方法测试了基于不同额定电压直流电机和减速比构建的微泵工作性能;并以该微泵为核心部件建立了自动细胞培养回路,开展了空间实验常用细胞系MG63和血管内皮细胞的中长周期培养试验。结果零背压下微泵流量与驱动电压呈良好的线性关系,以额定电压8 V(16 V)电机构建的微泵,电压由6 V增至10 V(8 V增至12 V)时,流量由0.29 m L/min增至0.67 m L/min(2.63 m L/min增至4.95 m L/min);持续15 d的测量表明微泵工作稳定性很好。MG63和血管内皮细胞均可在以该微泵为核心的自动细胞培养回路中正常生长达15 d。结论该泵头分体式直流电机驱动微泵工作污染风险低,流量易调,可满足我国空间自动化细胞培养系统搭建的需求。
[Abstract]:Objective to establish a miniaturized, integrated and automated cell culture system suitable for the study of cell biology in aerospace medicine in China. The low cost and self-control function of the pump head driven by the split DC motor peristaltic micro-pump. Methods the performance of the micro-pump based on different rated voltage DC motor and deceleration ratio was tested. With the micropump as the core component, the automatic cell culture loop was established, and the medium and long period culture experiments of the usual cell line MG63 and vascular endothelial cells were carried out. Results there was a good linear relationship between the flow rate and the driving voltage of the micropump under zero back pressure. A micropump constructed from a rated voltage of 8 V to 16 V) when the voltage is increased from 6 V to 10 V / 8 V to 12 V), The flow rate increased from 0.29 m L/min to 0.67 m L/min(2.63 m L/min to 4.95 mL / min, and the measurements for 15 days showed that the stability of the micropump was very good. MG63 and vascular endothelial cells could grow up to 15% in the automatic cell culture loop with the micropump as the core. Conclusion the working pollution risk of the micro-pump driven by the split DC motor of the pump head is low. The flow rate is easy to be adjusted, which can meet the need of building the space automation cell culture system in China.
【作者单位】： 中国航天员科研训练中心航天医学基础与应用国家重点实验室;
【基金】：航天医学基础与应用国家重点实验室资助项目(SMFA13A03,SMFA15A02)
【分类号】：R85
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本文编号：1673956