雌激素对模拟失重及飞船舱内噪声下豚鼠内耳Caspase-3表达的影响

发布时间：2018-04-01 05:37

本文选题：失重　切入点：噪声　出处：《听力学及言语疾病杂志》2013年05期

【摘要】：目的探讨失重及飞船舱内噪声复合因素对豚鼠听功能及内耳凋亡标志物Caspase-3表达的影响,并观察雌激素对上述复合因素暴露后豚鼠听功能损伤及内耳细胞凋亡的防治作用。方法 36只豚鼠随机分为4组,正常对照组6只(对照组),失重+噪声组(A组)、雌激素治疗组(B组)、雌激素预防组(C组)各10只。各实验组动物均后肢悬吊模拟失重,同时模拟飞船舱内噪声暴露5天,B组暴露过程中每天肌注苯甲酸雌二醇0.08mg/kg,首剂加倍,至暴露结束后3天;C组暴露前3天开始肌注苯甲酸雌二醇0.08mg/kg,首剂加倍,持续3天。对照组不给任何处理。于实验前、暴露后即刻和恢复3天时行听性脑干反应测试,免疫组织化学染色方法观察凋亡标志物Caspase-3在内耳毛细胞、血管纹及螺旋神经节细胞上的表达。结果暴露后当天,B组ABR反应阈低于A组和C组(P0.05);暴露前后B组ABR阈移也低于A组和C组(P0.05);恢复前后,C组ABR阈移高于A组低于B组(P0.05)。对照组毛细胞、血管纹和螺旋神经节Caspase-3表达均为阴性;A、B、C组在暴露后即刻、恢复3天时,毛细胞、血管纹和螺旋神经节上Caspase-3的表达均为阳性;暴露后即刻,B组毛细胞和血管纹Caspase-3表达较弱,而C组表达较强;恢复3天后,A、B、C三组毛细胞、血管纹和螺旋神经节Caspase-3表达仍为阳性,C组表达较弱。结论使用雌激素治疗可有效减少失重和噪声复合作用暴露后即刻的听力损失和内耳细胞凋亡;而预防性使用雌激素对暴露后即刻的听力损伤无保护作用,但在恢复过程中可促进其恢复,减少内耳细胞凋亡。
[Abstract]:Objective to investigate the effects of weightlessness and noise in the cabin on auditory function and Caspase-3 expression of apoptotic markers in inner ear of guinea pigs. To observe the protective effect of estrogen on auditory function injury and inner ear cell apoptosis in guinea pigs after exposure to the above complex factors, 36 guinea pigs were randomly divided into 4 groups. Six normal control groups (control group, weightlessness noise group, group A, estrogen treatment group, estrogen prevention group, group C), 10 rats in each group. At the same time, during the 5 days of noise exposure in flight cabin, estradiol benzoate was injected intramuscularly into group B (0.08 mg / kg), the first dose was doubled, and the first dose was doubled 3 days before exposure to Estradiol benzoate (0.08 mg / kg) in group C 3 days after exposure. After 3 days of treatment, the control group was given no treatment. Auditory brainstem response was measured immediately after exposure and 3 days after recovery. The apoptotic marker Caspase-3 was observed in hair cells of inner ear by immunohistochemical staining. Results the ABR response threshold of group B was lower than that of group A and group C on the day of exposure, the threshold shift of ABR in group B was also lower than that in group A and group C before and after exposure, and the threshold shift of ABR in group C was higher than that in group A before and after recovery. Group B, P0.05. the control group, hair cells, The expression of Caspase-3 in hair cell, stria vascularis and spiral ganglion were all positive in group C with negative expression of Caspase-3 in stria vascularis and spiral ganglion immediately after exposure, but Caspase-3 expression in hair cell and stria vascularis was weak in group B immediately after exposure. After 3 days of recovery, the hair cells in group C were significantly higher than those in group C, and that in group C was higher than that in group C (P < 0.05). The expression of Caspase-3 in stria vascularis and spiral ganglion was still weak in group C. Conclusion estrogen therapy can effectively reduce hearing loss and apoptosis of inner ear cells immediately after exposure to combined effects of weightlessness and noise. However, prophylactic use of estrogen had no protective effect on hearing loss immediately after exposure, but it could promote the recovery and decrease the apoptosis of inner ear cells during the recovery process.
【作者单位】：解放军306医院耳鼻咽喉头颈外科;北京大学解放军306医院教学医院;解放军306医院病理科;中国航天员科研训练中心;
【基金】：全军十一五医药卫生科技攻关科研项目(08G059)资助
【分类号】：R85

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