缺氧预处理对模拟失重大鼠比目鱼肌萎缩的预防

发布时间：2018-04-12 13:54

本文选题：失重模拟 + 缺氧预处理　；参考：《航天医学与医学工程》2006年04期

【摘要】：目的了解缺氧预处理对失重状态下发生的骨骼肌萎缩是否有预防作用。方法采用大鼠尾部悬吊法建立模拟失重动物模型,将雌性SD大鼠按体重配对原则随机分为对照组(CON)、尾部悬吊组(SUS)及缺氧预处理+尾部悬吊(HCP+SUS)组。首先,对HCP+SUS组进行1周的缺氧预处理。处理完成后,将SUS及HCP+SUS组大鼠尾部悬吊2周。试验结束处死3组大鼠,取双后肢比目鱼肌,分别称重。将部分比目鱼肌制成匀浆,取上清液用于超氧化物歧化酶(SOD)、丙二醛(M DA)的检测。结果尾部悬吊2周后,SUS组大鼠双后肢比目鱼肌湿重明显低于CON及HCP+SUS组(P0.01)。与SUS组相比,HCP+SUS组比目鱼肌SOD活性明显升高(P0.05),M DA水平明显降低(P0.05)。结论缺氧预处理可提高大鼠骨骼肌的抗氧化能力,对失重状态下发生的骨骼肌萎缩有预防作用。
[Abstract]:Objective to investigate the preventive effect of hypoxia preconditioning on skeletal muscle atrophy in weightlessness.Methods the animal model of simulated weightlessness was established by tail suspension. Female SD rats were randomly divided into control group, tail suspension group (SUSS) and hypoxic preconditioning tail suspension (HCP SUSS) group according to the principle of body weight pairing.First, anoxic preconditioning was performed for 1 week in HCP SUS group.After treatment, the tail of SUS and HCP SUS rats were suspended for 2 weeks.Three groups of rats were killed at the end of the experiment.Some soleus muscles were made into homogenate and superoxide dismutase (SOD) and malondialdehyde (MDA) were used in the detection of superoxide dismutase (SOD) and malondialdehyde (MDA) in superoxide dismutase (SOD).Results the wet weight of soleus muscle in SUS group was significantly lower than that in CON and HCP SUS groups after 2 weeks of tail suspension.Compared with the SUS group, the activity of SOD in the soleus muscle in the HCP SUS group was significantly higher than that in the SUS group.Conclusion hypoxia preconditioning can improve the antioxidant ability of skeletal muscle and prevent skeletal muscle atrophy under weightlessness.
【作者单位】：成都体育学院运动医学系四川大学华西第二医院四川大学华西第二医院成都体育学院运动医学系成都体育学院运动医学系成都体育学院运动医学系成都体育学院运动医学系
【分类号】：R85

【相似文献】