火器—冻复合伤后早期血液心肌酶组合变化研究

发布时间：2018-04-18 00:28

本文选题：火器-冻复合伤 + 冻伤　；参考：《兰州大学》2011年硕士论文

【摘要】：目的：基于寒冷地区发生局部战争或恐怖袭击或其他突发事件的客观事实,瞄准寒冷地区防御作战、执行非战争任务,建立火器-冻、冻-火器复合伤动物模型,揭示寒冷地区火器-冻、冻-火器复合伤后早期血液心肌酶组合改变特点和规律。为提高寒冷地区火器-冻、冻-火器复合伤救护水平与制定具体措施,减少因血液心肌酶组合改变所带来的伤亡奠定基础。方法：将30只普通级日本大耳白兔随机分为五组：A火器-冻伤组(n=6),B冻-火器伤组(n=6),C火器伤组(n=6),D冻伤组(n=6),E未受伤对照组(n=6)。各组制备相应的动物模型；(75±50)min后经颈动脉测压管采血测肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、α-羟丁酸脱氢酶(α-HBDH),分析各组受伤后早期血液心肌酶组合变化。结果：各组动物模型构建成功。A组与对照组E相比：CK、CK-MB、LDH、AST、ALT、α-HBDH升高有非常显著性差异(P＜0.01)；B组与对照组E相比：CK、CK-MB、LDH、AST、ALT、α-HBDH升高有显著性差异(P＜0.05)；C组与对照组E相比：CK、CK-MB、LDH、AST、α-HBDH升高有显著性差异(P＜0.05),ALT虽无显著性差异(P＜0.05),但ALT有上升趋势；D组与对照组E相比：CK、CK-MB、LDH、AST、ALT、α-HBDH虽无显著性差异(P＜0.05),但都有上升趋势。A组和B组相比：CK、CK-MB、LDH、AST、ALT、α-HBDH升高有显著性差异(P＜0.05)。A、B组与C组相比：CK、CK-MB、LDH、AST、ALT、α-HBDH升高有显著性差异(P＜0.05)。A、B组与D组相比：CK、CK-MB、LDH、AST、ALT、α-HBDH升高有显著性差异(P＜0.05)。C组与D组相比：CK、CK-MB、LDH、AST、α-HBDH升高有显著性差异(P＜0.05),ALT虽无显著性差异(P＞0.05),但ALT有上升趋势。结论：火器-冻、冻-火器复合伤不仅有火器伤因素作用,还有低温环境因素作用,两种因素同时作用。与单一伤相比,火器-冻复合伤后血清心肌酶升高最明显,组织损伤最为严重；冻-火器复合伤后血清心肌酶升高明显,提示组织损伤较为严重；火器伤后血清心肌酶升高,提示组织损伤严重；冻伤后血清心肌酶有升高趋势,提示组织有轻微损伤。
[Abstract]:Objective: based on the objective facts of local war or terrorist attack or other unexpected events in cold areas, aiming at the defense operations in cold regions, carrying out non-war tasks, establishing the animal model of firearms-frozen, frost-firearm combined injury,To reveal the characteristics and regularity of the changes of myocardial enzyme combination in the early stage after firearm-frozen and frozen-firearm combined injury in cold area.In order to improve the rescue level of firearm-frozen, frozen-firearm combined injury and make specific measures to reduce the casualties caused by changes of blood myocardial enzyme combination.Methods: thirty Japanese white rabbits of general grade were randomly divided into five groups: 1. A firearm-frostbite group (n = 6) and a firearm injury group (n = 6), a firearm injury group (n = 6) and a firearm injury group (n = 6). The frostbite group (n = 6) was divided into 5 groups (n = 5) and the control group (n = 6) was uninjured.In each group, 75 卤50)min blood samples were collected to measure creatine kinase (CK), creatine kinase isoenzyme (CK-MBN), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (alt), 伪 -hydroxybutyrate dehydrogenase (伪 -HBDH).The changes of myocardial enzyme composition in the early period after injury were analyzed.Results: compared with the control group E, there were very significant differences in the elevation of ALT, 伪 -HBDH and 伪 -HBDH between the two groups (P < 0.01). There were significant differences in the elevation of ALT, 伪 -HBDH and 伪 -HBDH between the two groups (P < 0.05) and the control group E (P < 0.05).Although there was no significant difference (P < 0.05), there was an increasing trend of ALT in group D (P < 0.05). Although there was no significant difference in ALT between group D and group E, 伪 -HBDH had no significant difference (P < 0.05), but there was an increasing trend in group A and group B. there was significant difference in the elevation of 伪 -HBDH between group A and group B (P < 0.05). There was a significant difference between group A and group C (P < 0.05). The increase of 伪 -HBDH in group A was significantly higher than that in group C (P < 0.05).The difference was significant (P < 0.05). Compared with D group, there was a significant difference in the elevation of 伪 -HBDH and 伪 -HBDH between group A and group D. There was no significant difference in the elevation of 伪 -HBDH between group C and group D (P < 0.05), but there was a trend of increase in ALT in group C and group D (P < 0.05), but there was no significant difference in the elevation of 伪 -HBDH between group A and group D (P < 0.05), but there was a trend of increase in ALT in group C (P < 0.05) compared with that in group D (P < 0.05), but there was no significant difference between group A and group D (P > 0.05).Conclusion: firearm-frozen, frozen-firearm combined injury not only has the effect of firearm injury, but also has the effect of low temperature environment factor, two kinds of factors acting at the same time.Compared with single injury, the increase of serum myocardial enzyme was the most obvious and the tissue injury was the most serious after firearm combined injury, and the increase of serum myocardial enzyme after firearm injury was significant, which indicated that the tissue injury was more serious, and the level of serum myocardial enzyme was higher after firearm injury.The results indicated that the tissue injury was serious and the serum myocardial enzyme increased after frostbite, suggesting slight tissue injury.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R826.1

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