中药干预模拟失重大鼠股骨外源钙沉积研究

发布时间：2018-04-18 04:39

本文选题：Ca + 中药　；参考：《中国中药杂志》2009年09期

【摘要】：目的:研究中药复方对模拟失重大鼠承重骨对外源钙摄取的干预作用。方法:雄性Wistar大鼠随机分为3组,空白组、模型组、中药组。尾吊模拟失重,中药复方(含熟地黄、怀牛膝、黄芪、当归、牡蛎醋酸水解物等)704 g.L-1水煎剂2.5mL灌胃;适应期1周,模拟失重期3周,于模拟失重第11天一次性灌胃给予一定量41Ca示踪剂。实验结束取股骨,加速器质谱法(Accelerator Mass Spectrometry,AMS)分析骨41Ca/40Ca值,电感耦合等离子体光谱法(Inductively Coupled Plasma-AtomicEmission Spectroscopy,ICP-AES)分析骨钙总量,计算股骨41Ca沉积量、股骨41Ca沉积率。结果:空白、模型、中药3组股骨41Ca/40Ca丰度比分别为(5.03±0.55),(3.82±0.29),(5.73±0.40)×10-9,模型组较空白组显著降低(P0.001),中药组较空白组显著升高(P0.05);空白、模型、中药3组股骨41Ca沉积量分别为(7.39±0.90),(4.52±0.40),(7.45±0.89)×10-7mg,沉积率分别为(0.819±0.100),(0.501±0.045),(0.826±0.098)%,模型组股骨41Ca沉积量和沉积率均较空白组显著降低(P0.001),中药组较空白组变化不显著,较模型组显著增高(P0.001)。结论:尾吊模拟失重可造成大鼠承重骨外源钙沉积障碍,中药可较好对抗这种情况;失重骨质疏松可能存在外源钙沉积减少以外的多种机制。
[Abstract]:Aim: to study the effect of Chinese medicine compound on exogenous calcium uptake in weight-bearing bone of simulated weightlessness rats.Methods: male Wistar rats were randomly divided into 3 groups: blank group, model group and traditional Chinese medicine group.Tail suspension simulated weightlessness, traditional Chinese medicine compound (including Radix Rehmanniae, Achyranthes bidentata, Radix Astragali, Angelica sinensis, oyster acetic acid hydrolysate, etc.) 2.5mL, the adaptation period was 1 week, and the simulated weightlessness period was 3 weeks.A certain amount of 41Ca tracer was given once on the 11th day of simulated weightlessness.At the end of the experiment, the femur was analyzed by Accelerator Mass Spectrometry (AMS), and the total bone calcium was analyzed by inductively Coupled Plasma-AtomicEmission spectroscopy (ICP-AESS-MS). The deposition of 41Ca in femur and 41Ca deposition rate in femur were calculated.Results: the 41Ca/40Ca abundance ratio of femur in the blank, model and TCM groups was 5.03 卤0.55, 3.82 卤0.29, 5.73 卤0.40) 脳 10 ~ (-9), respectively. Compared with the blank group, the 41Ca/40Ca abundance ratio of the model group was significantly lower than that of the blank group, and that of the traditional Chinese medicine group was significantly higher than that of the blank group, while the blank, the model group was significantly higher than that of the blank group.The deposition amount of 41Ca in the femur of the three groups were 7.39 卤0.90, 4.52 卤0.40, 7.45 卤0.89) 脳 10 ~ (-7) mg, and the deposition rates were 0.819 卤0.100 卤0.501 卤0.045, 0.826 卤0.098, respectively. The 41Ca deposition and deposition rate of the femur in the model group were significantly lower than those in the blank group (P 0.001), but the changes in the traditional Chinese medicine group were not significant compared with the blank group, and significantly higher than that in the model group.Conclusion: tail suspension simulated weightlessness can cause exogenous calcium deposition obstacle in load-bearing bone of rats, Chinese medicine can better resist this situation, and weightlessness osteoporosis may have some mechanisms other than the reduction of exogenous calcium deposition.
【作者单位】：北京中医药大学基础医学院;天津中医药大学中药学院;中国原子能科学研究院;
【基金】：国家自然科学基金项目(30500663) 2006年教育部优秀人才支持计划项目(NCET-06-0124) 2006年北京市科技新星(A)类计划项目(2006A49) 国家基础科学人才培养基金(20070110)
【分类号】：R85

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