封闭负压引流对猪爆炸伤创面毛细血管新生的影响

发布时间：2018-04-20 21:06

本文选题：感染 + 伤口愈合　；参考：《中华烧伤杂志》2007年04期

【摘要】：目的观察封闭负压引流(VAC)技术对猪爆炸伤感染创面肉芽组织毛细血管新生的影响。方法用电雷管在4只小白家猪双侧肩胛及双侧臀部各造成1个面积为(7.3±1.0)cm~2爆炸伤创面,按完全随机化方法分为对照组(8个创面)和治疗组(8个创面)。两组均在伤后2 d行创面处理。对照组:创面清创后覆盖单层油纱;治疗组:清创后用VAC治疗。各组均于处置前及处置后1、3、6 d切取2 mm×2 mm×2 mm的创面组织行病理切片。光学显微镜下观察周皮细胞的分化、Image C软件行毛细血管管腔面积计算及图像处理。结果对照组处置前及处置后1、3 d,创面毛细血管较少,且血管内皮细胞周围缺少周皮细胞包裹;处置后6 d毛细血管增多,有少量周皮细胞包裹。治疗组处置后1 d毛细血管增加,并有充足的周皮细胞包裹;此后有周皮细胞包裹的毛细血管逐渐增多。治疗组处置后1、3、6 d创面内结蛋白阳性颗粒吸光度值(2569±330、3984±377、9611±960)均高于对照组(91±54、199±85、1552±298,P0.01)。对照组处置后1、3、6 d毛细血管管腔总面积较小,分别为[(59±36)、(250±70)、(938±287)·μm~2],均小于治疗组[(818±234)、(4518±1080)、(9058±1656)μm~2,P0.01]。结论与常规换药相比,VAC不仅能使猪皮肤软组织爆炸伤感染创面肉芽组织内新生毛细血管形成,还能促进周皮细胞分化,增加毛细血管管腔面积。
[Abstract]:Objective to observe the effect of negative pressure drainage (VAC) technique on capillary neovascularization in granulation tissue of wound infected with explosive wound in pigs. Methods the electric detonator caused 1 wound area of 7. 3 卤1.0)cm~2 explosive wound on both shoulder blades and buttocks of 4 piglets. According to complete randomization, it was divided into two groups: control group (8 wounds) and treatment group (8 wounds). Wound management was performed 2 days after injury in both groups. The control group was covered with single layer gauze after wound debridement, and the treatment group was treated with VAC after debridement. The wound tissues of 2 mm 脳 2 mm 脳 2 mm were taken from each group before and 6 days after treatment. The differentiation of pericyte was observed under optical microscope. The area of capillary lumen was calculated and the image was processed by image C software. Results in the control group, there were fewer capillaries in the wound before and 1 day after treatment, and there were no pericytes around the vascular endothelial cells, but the capillaries increased and a few pericytes were encapsulated in the control group at 6 days after treatment. In the treatment group, the capillaries increased 1 day after treatment, and there were plenty of pericarp cells in the treatment group, and the capillary capillaries wrapped by pericarp cells gradually increased after treatment. The absorbance value of desmin positive granules in the treatment group was 2569 卤330U 3984 卤377U 9611 卤96011 卤9606 days after treatment, which was higher than that in the control group (91 卤54199 卤851552 卤298P0.01a). The total area of capillary lumen in the control group was smaller than that in the treatment group on the 1st day and 3rd day after treatment, which was [59 卤36, 250 卤70, 938 卤287], and was lower than that in the treatment group [818 卤234, 4518 卤1080, 9058 卤1656, 渭 m2P0.01, respectively]. Conclusion VAC can not only induce the formation of new capillaries in granulation tissue, but also promote the differentiation of pericarp cells and increase the area of capillary lumen.
【作者单位】：第四军医大学唐都医院烧伤整形科;
【分类号】：R641;R82

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