一种基于表面等离子体共振技术的生物小分子检测方法

发布时间：2018-04-28 02:54

本文选题：表面等离子体 + 共振　；参考：《航天医学与医学工程》2005年02期

【摘要】：目的在以表面等离子体共振生物传感器为核心的便携式生物分子在线分析系统的基础上,建立一种小分子检测方法,满足航天员体液在线检测需求。方法建立小分子物质2,4二硝基苯肼(DNPH)的检测模型。用DNP BSA制备生物分子膜;采用抑制检测法,得到检测区间及标准曲线;对检测的可重复性和特异性进行了实验。结果1)DNPH检测的线性范围为7.8ng/ml～2μg/ml,其检测下限为2.5ng/ml;2)生物分子膜的制备及传感器的再生方法能满足多样品测定的需求。结论本方法灵敏、快速、简捷地实现了小分子物质检测,且重复性好、可信度高,在航天医学检测中具有广泛的应用前景。
[Abstract]:Aim based on the portable on-line analysis system of biomolecules based on the surface plasmon resonance biosensor, a small molecular detection method was established to meet the requirements of the on-line detection of astronauts' body fluids. Methods the detection model of DNPH was established for the small molecular substance 2o 4 dinitrophenylhydrazide (DNPH). The biomolecular membrane was prepared by DNP BSA, the detection interval and standard curve were obtained by inhibition detection method, and the repeatability and specificity of the detection were tested. Results the linear range of 1)DNPH detection was 7.8ng/ml~2 渭 g / ml, the detection limit was 2.5 ng / ml ~ (-2) the preparation of biomolecular membrane and the regeneration method of biosensor could meet the needs of multi-sample determination. Conclusion the method is sensitive, rapid and simple for the detection of small molecular matter, and has good reproducibility and high reliability. It has a wide application prospect in space medicine detection.
【作者单位】：航天医学工程研究所航天医学工程研究所航天医学工程研究所航天医学工程研究所航天医学工程研究所航天医学工程研究所
【基金】：总装备部重点实验技术研究课题资助
【分类号】：R85

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