模拟失重对大鼠肠黏膜NF-κB表达的影响

发布时间：2018-05-16 23:44

本文选题：核因子-κB + 模拟失重　；参考：《世界华人消化杂志》2008年29期

【摘要】：目的:探讨模拟失重条件下健康大鼠肠道黏膜组织中核因子-κB(NF-κB)的表达变化及其意义.方法:成年Wistar大鼠80只,随机分为10组,按模拟失重时相分别为0.5、1、2、7、21d和相应的对照组.采用尾悬吊法建立模拟失重动物模型.免疫组织化学法检测肠道黏膜组织中NF-κB的表达水平.结果:0.5、1、2、7、21d模拟失重组大鼠肠道组织NF-κB表达水平均显著高于相应对照组(10.11%±3.29%vs5.50%±1.92%,22.00%±5.31%vs6.50%±2.32%,25.50%±4.11%vs8.75%±6.36%,21.50%±3.02%vs6.75%±2.12%,10.87%±2.64%vs5.62%±2.13%,均P0.01),模拟失重0.5d组肠道组织NF-κB表达开始升高,2d组达高峰,随模拟失重时间的延长,NF-κB表达水平依次下降,21d组仍然高于相应对照组.结论:尾悬吊模拟失重大鼠肠道组织NF-κB表达水平明显上调,提示肠道组织NF-κB表达变化与失重应激反应和失重耐受有密切关系.达水平明显上调,提示肠道组织NF-κB表达变化与失重应激反应和失重耐受有密切关系.
[Abstract]:Aim: to investigate the expression and significance of nuclear factor- 魏 B (NF- 魏 B) NF- 魏 B in intestinal mucosa of healthy rats under simulated weightlessness. Methods: 80 adult Wistar rats were randomly divided into 10 groups. An animal model of simulated weightlessness was established by tail suspension. The expression of NF- 魏 B in intestinal mucosa was detected by immunohistochemistry. Results the expression level of NF- 魏 B in intestinal tissue of rats with simulated loss was significantly higher than that of control group (10.11% 卤3.29 vs 5.50% 卤1.92 卤22.00% 卤2.31 卤25.50% 卤2.32 卤25.50% 卤4.11 vs 8.75% 卤6.36V 21.50% 卤3.02 vs 6.75% 卤2.64 vs 5.62% 卤2.13). With the prolongation of simulated weightlessness, the expression level of NF- 魏 B decreased in turn. Conclusion: the expression level of NF- 魏 B in intestinal tissue of simulated weightlessness rats with tail suspension was significantly up-regulated, suggesting that the changes of NF- 魏 B expression in intestinal tissue were closely related to weightlessness stress response and weightlessness tolerance. The expression of NF- 魏 B in intestinal tissue was significantly up-regulated, suggesting that there was a close relationship between NF- 魏 B expression and weightlessness stress response and weightlessness tolerance.
【作者单位】：中国人民解放军第三军医大学西南医院普通外科;中国人民解放军第306医院;
【分类号】：R85

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