模拟月尘致大鼠肺脏急性损伤病理学变化

发布时间：2018-06-07 14:12

本文选题：模拟月尘 + 肺　；参考：《航天医学与医学工程》2017年02期

【摘要】：目的探讨模拟月尘(lunar soil simulant,LSS)对大鼠肺脏急性损伤的病理学改变。方法采用粒径在5μm以下的CAS-1模拟月壤作为真实月壤的替代物。用无菌生理盐水配制模拟月尘混悬液。通过气管滴注染毒法制备模拟月尘的动物模型,同时与PM2.5及生理盐水组对照,观察染尘不同时期大鼠肺脏的超微结构变化。结果 LSS高剂量组(灌注量1 m L,含7 mg LSS)4 h图片可见肺泡间隔增厚和中性粒细胞浸润;24 h图片可见肺泡间隔增厚和血管内中性粒细胞浸润,以及血管和支气管周围炎。LSS低剂量组(灌注量1 m L,含0.7 mg LSS)4 h图片未见组织明显病变;24 h图片可见肺泡隔轻微增厚,支气管周围有中性粒细胞浸润但比LSS高剂量组轻。PM2.5组4 h和24 h图片均未观察到炎症、水肿。结论 LSS可能具有比PM2.5更强的肺毒性,可引起机体肺实质损伤,产生炎症性病变。
[Abstract]:Objective to investigate the pathological changes of acute lung injury induced by simulated lunar soil Simulant LSS in rats. Methods CAS-1 with particle size less than 5 渭 m was used to simulate lunar soil as a substitute for real lunar soil. The simulated lunar dust suspension was prepared with aseptic saline. The animal model of simulated lunar dust was established by trachea instillation and compared with PM2.5 and normal saline groups to observe the ultrastructural changes of lung in rats at different stages of exposure to dust. Results the alveolar septum thickening and neutrophil infiltration were observed in the high dose LSS group (perfusion volume 1 mL, including 7 mg LSS)4 h images). The alveolar septal thickening and intravascular neutrophil infiltration were observed in 24 h images. The alveolar septum was slightly thickened in vascular and peribronchitis 路LSS low-dose group (perfusion volume 1 mL, including 0.7 mg LSS)4 h images). Infiltration of neutrophils around the bronchus but no inflammation and edema were observed at 4 h and 24 h in the PM2.5 group, which was lighter than that in the high dose group of LSS. Conclusion LSS may have more pulmonary toxicity than PM2.5, which can cause lung parenchyma injury and inflammatory lesion.
【作者单位】：沈阳医学院基础医学院;沈阳化工研究院有限公司;辽宁何氏医学院;
【分类号】：R85

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