ACTN3、ADRA2A、睫状神经营养因子基因多态性与士兵速度、耐力、力量素质的相关性

发布时间：2018-06-18 04:01

本文选题：辅肌动蛋白 + 睫状神经营养因子　；参考：《中国组织工程研究与临床康复》2008年37期

【摘要】：背景:与体能素质有关的基因研究表明:辅肌动蛋白3基因(ACTN3)、α2A肾上腺素能受体基因(ADRA2A)和睫状神经营养因子(CNTF)基因3种基因分别与个体的速度素质、耐力素质和力量素质可能相关。目的:检测中国汉族男性新入伍军人ACTN3、ADRA2A和CNTF基因的遗传多态性,分析ACTN3、ADRA2A、CNTF基因多态性分别与速度、耐力和力量素质的相关性。设计:调查分析。时间及地点:实验于2007-10在解放军第四军医大学基因实验室完成。对象:分别从某两战区3个步兵师随机选取的2007年度中国汉族男性新入伍军人326名,平均年龄(18.82±0.88)岁。方法:入选326名士兵均无训练史,113名进行18周短跑训练,测试训练后的百米成绩。108名进行18周的有氧耐力训练,测试训练后5000m跑的成绩。105名进行18周常规新兵入伍力量性训练,测试训练后的握力体质量指数。主要观察指标:①用荧光定量聚合酶链反应法分析ACTN3基因的多态性。②DNA测序分析ADRA2A基因的G6412C、T6623A、C6645C3个位点的多态性。③用荧光定量聚合酶链反应法分析CNTF基因的多态性,分析握力体质量指数与基因多态性的关系。④3种基因分别与速度素质、耐力素质和力量素质的相关分析。结果:①3种基因的多态位点分布频率均符合Hardy-Weinberg平衡。ACTN3基因C/T、C/C基因型显著性多于T/T基因型(P0.05)。ACTN3基因多态性与速度素质有相关性,T/T纯合型速度素质优于C/C、C/T型。②ADRA2A基因位点T6623A多态性与耐力素质相关,A/A基因型耐力素质显著性高于A/G基因型(P0.05),G/G基因型和A/G、A/A基因型的耐力素质差异无显著性意义(P0.05)。未发现G6412C、C6645C单点多态性与耐力素质有明显的相关性。③CNTF基因G/G基因型群体显著性多于A/G、A/A。CNTF多态性与力量素质未见显著性相关性(P=0.16)。结论:①ACTN3基因多态性可作为预测个体速度素质的基因标记。②ADRA2A基因位点T6623A多态性可作为耐力素质的基因标记,而位点G6412C和C6645C多态性不是预测个体耐力素质的基因标记。③CNTF基因可以作为CNTF基因A/G多态频率分布的代表,其多态性不能作为中国汉族男性新入伍军人预测个体力量素质的基因标记。
[Abstract]:Background: studies on genes related to physical fitness have shown that the three genes of coactin 3 gene (ACTN3), 伪 2A adrenoceptor gene (ADRA2A) and ciliary neurotrophic factor (ciliary neurotrophic factor CNTF) gene are associated with the individual's speed quality, respectively. Endurance and strength may be related. Objective: to detect the genetic polymorphisms of ACTN3ARA2A and CNTF genes in Chinese Han male recruits, and to analyze the relationship between ACTN3ARA2ANTF gene polymorphism and speed, endurance and strength quality. Design: investigation and analysis. Time and place: the experiment was carried out in the Genetics Laboratory of the fourth military Medical University of PLA in October 2007. Participants: 326 Chinese Han male recruits were randomly selected from 3 infantry divisions in two war zones in 2007, with an average age of 18.82 卤0.88 years. Methods: 113 of 326 soldiers who had no history of training were trained in sprint for 18 weeks. 108 of 100 meters were tested for aerobic endurance training for 18 weeks, and 105 for 5 000 m running after 18 weeks of regular strength training. The body mass index of grip strength was tested after training. Main outcome measures: the polymorphism of ACTN3 gene was analyzed by fluorescence quantitative polymerase chain reaction (FQPCR). 2 DNA sequencing was used to analyze the polymorphism of ACTN3 gene in ADRA2A gene. The polymorphism of C6645C3 locus of ADRA2A gene was analyzed by fluorescence quantitative polymerase chain reaction (FQ-PCR), and the polymorphism of CNTF gene was analyzed by fluorescence quantitative polymerase chain reaction (FQ-PCR). To analyze the relationship between grip strength body mass index and gene polymorphism. 43 genes were analyzed respectively with speed quality endurance quality and strength quality. Results the frequencies of polymorphic loci of the 13 genes were all consistent with Hardy-Weinberg equilibrium. ACTN3 gene C / T / C / C genotype was significantly higher than that of T / T genotype (P 0.05). ACTN3 gene polymorphism was correlated with the speed quality of T / T homozygous type speed quality was superior to that of C / C / C / T / 2 ADRA2A gene. Because of T6623A polymorphism and endurance quality, there was no significant difference in endurance quality of A / A genotype than that of A / G genotype P0.05G / G genotype and A / G / A / A genotype. There was no significant correlation between G6412 C6645C single point polymorphism and endurance diathesis. 3There was no significant correlation between the Gr / G genotype population of CNTF gene and the strength quality of A / G / A / CNTF genotype. There was no significant correlation between G6412 C6645C polymorphism and endurance quality. Conclusion the T6623A polymorphism of ACTN3 gene can be used as a gene marker to predict individual speed quality. 2ADRA2A gene locus T6623A polymorphism can be used as a genetic marker of endurance quality. The polymorphism of G6412C and C6645C is not the marker of individual endurance quality. 3CNTF gene can be used as a representative of the frequency distribution of CNTF gene Ar / G polymorphism. The polymorphism can not be used as a genetic marker to predict the individual strength quality of Chinese male recruits.
【作者单位】：解放军第一五医院全军军事训练医学研究所;
【分类号】：R82

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