核因子κB在肺型氧中毒发病中的作用研究

发布时间：2018-06-21 02:15

本文选题：肺型氧中毒 + 高压氧　；参考：《第二军医大学》2006年硕士论文

【摘要】：在潜水高气压作业及临床高压氧(hyperbaric oxygen,HBO)治疗中,都涉及机体在高分压氧中暴露的问题。高分压氧的应用可以提高减压时惰性气体脱饱和速率,缩短减压时间,提高水下作业效率,并且对一些缺氧性疾病有明确的治疗效果,但是长时间吸入高分压氧又带来氧中毒的问题。氧中毒是机体吸入高于50kPa的氧气一定时间后,导致机体某些器官的结构与功能发生病理性变化而表现的病症。氧中毒分为眼型、脑型、和肺型三种类型,其中肺型氧中毒的主要表现为咳嗽、胸骨后疼痛、呼吸困难,形态学观察发现早期以炎症渗出为主,表现为肺部出现大面积充血、出血和水肿,炎细胞浸润。对于氧中毒的发病机理,至今仍未完全明了。核因子κB(NF-κB)是一个多功能核转录因子,具有广泛的生物学活性,激活后可促进细胞因子、粘附分子、趋化因子等基因转录,在应激、炎症和免疫反应等过程中发挥重要作用,成为近年来研究的热点。最近研究发现高分压氧能够导致NF-κB活化。肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)是受NF-κB调控表达的具有炎症介导活性的一种细胞因子,TNF-α的高表达可以造成肺损伤。本实验目的是通过研究NF-κB和TNF-α在氧中毒时的改变规律,确定NF-κB活化在肺型氧中毒发病中的作用,探讨肺型氧中毒的可能机制,为预防肺型氧中毒提供新的思路。本研究采用健康SD大鼠在密闭氧舱中吸入高压氧的方法建立肺型氧中毒模型。实验共分6组,分别是正常对照组、常氧高氮对照组、0.23MPa高压氧暴露2h、6h、
[Abstract]:In the treatment of high pressure diving and hyperbaric oxygen (HBO) treatment, it is involved in the exposure of the body to high partial pressure oxygen (HBO). The application of high partial pressure oxygen can improve the desaturation rate of inert gas during decompression, shorten the decompression time, improve the efficiency of underwater operation, and have definite therapeutic effect on some hypoxic diseases. But long-term inhalation of high partial pressure oxygen also leads to oxygen poisoning. Oxygen poisoning is a kind of disease caused by pathological changes in the structure and function of some organs after inhaling oxygen above 50 KPA for a certain time. Oxygen poisoning can be divided into three types: eye type, brain type, and lung type. The main manifestations of oxygen poisoning in pulmonary type are cough, pain after sternum, dyspnea. Bleeding and edema, inflammatory cell infiltration. The pathogenesis of oxygen poisoning has not been fully understood. NF-魏 B is a multifunctional nuclear transcription factor with a wide range of biological activities. Activation can promote transcription of cytokines, adhesion molecules, chemokines and other genes, and play an important role in stress, inflammation and immune response. It has become a hot topic in recent years. Recent studies have found that high partial pressure oxygen can lead to NF- 魏 B activation. Tumor necrosis factor-alpha (TNF- 伪) is a cytokine with inflammatory mediated activity regulated by NF- 魏 B, which can cause lung injury. The purpose of this study was to study the changes of NF- 魏 B and TNF- 伪 in oxygen poisoning, to determine the role of NF- 魏 B activation in the pathogenesis of pulmonary oxygen poisoning, to explore the possible mechanism of pulmonary oxygen poisoning, and to provide a new way of thinking for the prevention of pulmonary oxygen poisoning. A model of pulmonary oxygen poisoning was established by inhaling hyperbaric oxygen in a closed oxygen chamber in healthy SD rats. The experiment was divided into 6 groups: normal control group and normoxic hyperbaric oxygen control group with 0.23 MPA hyperbaric oxygen exposure for 2 h or 6 h, respectively.
【学位授予单位】：第二军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R84

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