低氧诱导法培养大鼠肺微血管内皮细胞

发布时间：2018-06-25 11:15

本文选题：缺氧 + 生长因子　；参考：《航天医学与医学工程》2000年05期

【摘要】：目的探讨大鼠肺微血管内皮细胞培养方法 ,并期望发展一种简便、实用的体外培养方法。方法用 7%O2 (4h/d ,3d)诱导培养猪肺动脉内皮细胞 ,然后收集该低氧诱导的培养基后加入大鼠肺边缘组织块 ,再培养微血管内皮细胞。结果加入低氧诱导培养基后 48h ,即有明显细胞增生 ;原代培养 1wk后 ,细胞呈典型的鹅卵石状连片生长 ,继续可以传代 3～ 4次。与对照方法相比 ,采用低氧诱导培养基法培养大鼠肺微血管内皮细胞 ,贴块后细胞增生反应较早出现、繁殖较快 ,细胞老化延迟 ,传代后细胞生长良好 ,3代以内细胞形态无明显退变。结论本文探讨的低氧诱导培养基方法 ,具有简便、实用的特点 ,可用于建立大鼠肺微血管内皮细胞的细胞模型 ,用于高原医学研究。
[Abstract]:Objective to explore the culture method of rat pulmonary microvascular endothelial cells and to develop a simple and practical culture method in vitro. Methods Porcine pulmonary artery endothelial cells were cultured with 7 脳 O2 (4h/d) for 3 days, then cultured in the hypoxic medium and then added into the lung marginal tissue mass of rats, and then cultured microvascular endothelial cells (MECs). Results 48 h after hypoxia induction medium, there were obvious proliferation of cells, and after primary culture of 1wk, the cells grew as typical pebbles and could continue to be subcultured for 3 ~ 4 times. Compared with the control method, the cultured rat pulmonary microvascular endothelial cells were cultured in hypoxic medium. The proliferation of the cells appeared earlier, the proliferation was faster, and the aging of the cells was delayed. After passage, there was no obvious degeneration within 3 ~ (th) passage. Conclusion the method of hypoxic induction medium is simple and practical. It can be used to establish the cell model of rat pulmonary microvascular endothelial cells and to be used in high altitude medical research.
【作者单位】：军事医学科学院卫生学环境医学研究所!天津300050 军事医学科学院卫生学环境医学研究所!天津300050 军事医学科学院卫生学环境医学研究所!天津300050 军事医学科学院卫生学环境医学研究所!天津300050 军事医学科学院卫生学环境医学研究所!天津300050
【分类号】：R85

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