模拟空间环境X射线和微重力对大鼠骨丢失的影响

发布时间：2018-06-26 13:17

本文选题：骨丢失 + 模拟微重力　；参考：《第三军医大学学报》2015年12期

【摘要】：目的探讨辐照和微重力对于骨代谢是否存在交互作用。方法 2月龄雄性Wistar大鼠分为正常对照组、X射线辐照组(X-ray组)、尾吊模型组(HLS组)及尾吊结合X射线辐照组(HLS+Xray组)。分别对HLS组和HLS+X-ray组进行4 Gy X射线全身辐照,对HLS+X-ray组进行尾吊实验来模拟空间环境下辐射和微重力的条件,检测骨密度、骨小梁的数目、骨小梁的分离程度、骨小梁的厚度、骨体积分数、结构模型指数等参数并检测了骨碱性磷酸酶(ALP)、酒石酸酸性磷酸酶(TRAP)、骨钙素(OCN)的及骨调节基因Rankl、Runx2、Nfatcl的表达变化,研究两种因素所引起的骨丢失是否有相应的交互作用。结果尾吊和X射线辐照在骨丢失方面引起了显著的变化,同正常对照组相比,HLS组和X-ray组的骨密度、骨小梁的数目、骨小梁的厚度、骨体积分数均表现为降低趋势(P0.05),HE染色显示HLS组、X-ray组和HLS+X-ray组中都出现骨小梁数量减少等特征,说明各个模型组都存在骨丢失现象,以HLS+X-ray组最为明显(P0.05)。Rankl、Nfatcl以及酒石酸酸性磷酸酶的表达在HLS组、Xray组及HLS+X-ray组均上升(P0.05)。碱性怜酸酶和骨钙素的表达在HLS组、X-ray组及HLS+Xray组均有所下降(P0.05)。而作为成骨细胞特异性表达的Runx2基因在HLS组和X-ray组却没有任何改变,在HLS+X-rays组发生了显著的下调(P0.05)。结论尾吊和X射线双因素能够引起骨密度更大的丢失,两者存在交互作用,破坏程度会更加严重。
[Abstract]:Objective to investigate the interaction between irradiation and microgravity on bone metabolism. Methods two month old male Wistar rats were divided into normal control group (X-ray group), tail suspension model group (HLS group) and tail suspension combined with X-ray irradiation group (HLS Xray group). The HLS group and HLS X-ray group were irradiated with 4 Gy X-ray. The tail suspension experiment was performed to simulate the radiation and microgravity conditions in the space environment. The bone density, the number of trabeculae and the degree of trabeculae separation were measured. Bone trabecular thickness, bone volume fraction, and structural model index were measured and the expression of bone alkaline phosphatase (ALP), tartrate acid phosphatase (trap), osteocalcin (OCN) and bone regulatory gene Ranklergen Runx2Nfatcl were detected. To study the interaction of bone loss caused by two factors. Results there were significant changes in bone loss caused by tail suspension and X-ray irradiation. The bone mineral density, the number of trabeculae and the thickness of trabecular bone in HLS and X-ray groups were significantly different from those in normal control group. Bone volume fraction showed a decreasing trend (P0.05) HE staining showed that the number of bone trabeculae decreased in HLS group X-ray group and HLS X-ray group, which indicated that bone loss existed in each model group. In HLS X-ray group, the expression of Nfatcl and tartrate acid phosphatase in HLS X-ray group and HLS X-ray group increased significantly (P0.05). The expression of alkaline pyrrolidase and osteocalcin in HLS group and HLS Xray group were decreased (P0.05). But the Runx2 gene, which was specifically expressed by osteoblasts, did not change in HLS group and X-ray group, but it was significantly down-regulated in HLS X-rays group (P0.05). Conclusion both tail suspension and X-ray can cause greater BMD loss, and there is interaction between them and the degree of damage will be more serious.
【作者单位】：第三军医大学研究生管理大队;成都军区昆明总院附属骨科医院全军创伤骨科研究所;昆明医科大学昆明总院临床分院;
【基金】：云南省科技厅支持项目(2010BC006)~~
【分类号】：R852.22

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