不同强度推拉动作对大鼠海马神经元bFGF表达的影响

发布时间：2018-07-03 04:18

本文选题：海马 + 神经元　；参考：《第四军医大学学报》2004年02期

【摘要】：目的 :采用动物离心机建立模拟推拉动作的动物模型 ,探讨“推拉效应”对大鼠海马神经元bFGF表达的影响 .方法 :健康雄性Wistar大鼠 4 0只 ,随机分为 1 0组 (对照组 ,三种强度暴露后的 30min,6h ,2 4h组 ) ,每组 4只 ,灌注固定 ,取大脑半球 ,切片后应用免疫组织化学染色及图像分析技术对大鼠海马各区神经元碱性成纤维细胞生长因子 (bFGF)表达进行检测 .结果 :对照组和± 2Gz暴露后的 30min组bFGF呈弱阳性表达 ;而± 6Gz和± 8Gz暴露后 30min的实验组可见大鼠海马神经元细胞bFGF表达开始增加 ,6h实验组表达明显增强 ,2 4h后表达下降但仍较正常对照组高 .与对照组比较 ,差异有显著性 (P 0 .0 5 ) .结论 :中、高强度的推拉动作可引起大鼠海马神经元bFGF表达增加
[Abstract]:Aim: to establish an animal model with animal centrifuge to simulate push-pull action and to investigate the effect of "push-pull effect" on the expression of bFGF in hippocampal neurons of rats. Methods: forty healthy male Wistar rats were randomly divided into 10 groups (control group, 30 min after exposure to three intensities for 6 h and 24 h), each group (4 rats) were infused and fixed, and the hemispheres were taken. The expression of basic fibroblast growth factor (bFGF) in hippocampal neurons of rats was detected by immunohistochemical staining and image analysis. Results: the expression of bFGF was weakly positive in control group and 卤2Gz exposed 30min group. The expression of bFGF in hippocampal neurons of rats exposed to 卤6Gz and 卤8Gz showed that the expression of bFGF began to increase at 6 h after exposure, and the expression of bFGF in experimental group decreased significantly after 24 h of exposure, but it was still higher than that in normal control group. Compared with the control group, the difference was significant (P 0. 05). Conclusion: the expression of bFGF in rat hippocampal neurons can be increased by high-intensity push-pull exercise.
【作者单位】：第四军医大学吉林军医学院医学实验技术教研室第四军医大学吉林军医学院医学实验技术教研室第四军医大学吉林军医学院医学实验技术教研室第四军医大学吉林军医学院医学实验技术教研室第四军医大学吉林军医学院医学实验技术教研室第四军医大学吉林军医学院医学实验技术教研室
【基金】：全军医药科研基金项目 (0 1MA2 0 2 )
【分类号】：R852

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