广州戊型病毒性肝炎暴发株和散发株部分序列比较

发布时间：2018-07-04 15:56

  本文选题：肝炎 + 戊型　； 参考：《中华传染病杂志》2006年02期

【摘要】：目的了解广州某部新兵连戊型病毒性肝炎(戊型肝炎)暴发流行的分子病毒学特征,并与当地散发毒株比较,以查找病原可能来源。方法用逆转录聚合酶链反应(RT-PCR)法,对抗HEV-IgM阳性的34例暴发性戊型肝炎及46例散发性戊型肝炎患者的血清和粪便标本进行HEVRNA检测,并对HEVRNA阳性标本的基因开放读码框(ORF)2部分片段进行克隆测序分析。结果34例暴发流行病例标本中检测出12株病毒,46例散发病例标本中检到2株。经克隆测序分析,各暴发毒株的核苷酸同源性为95.3%~100%;氨基酸同源性为94.0%~100%。且暴发毒株和散发毒株的核苷酸及氨基酸的同源性也较高,分别为95.3%~99.3%和94.0%~100%;暴发毒株和散发毒株与各型中的标准株相比,与Jap1株同源性最高,其核苷酸同源性为92.0%~95.3%,氨基酸同源性为96.0%~100.0%。进化树分析提示本次戊型肝炎暴发流行病毒株与戊型肝炎病毒基因Ⅳ型距离最近。结论本次戊型肝炎暴发流行的病原可能来于广州本地;广州地区戊型肝炎流行毒株属戊型肝炎基因型Ⅳ型。
[Abstract]:Objective to investigate the molecular virological characteristics of the outbreak of hepatitis E (hepatitis E) in recruits in Guangzhou, and to find out the possible source of the pathogen. Methods the serum and fecal samples of 34 fulminant hepatitis E patients and 46 sporadic hepatitis E patients with HEV-IgM positive were detected by reverse transcription-polymerase chain reaction (RT-PCR). The open reading frame (ORF) 2 fragments of HEV RNA positive samples were cloned and sequenced. Results 12 strains of virus were detected in 34 outbreak cases and 2 of 46 sporadic cases were detected. The nucleotide homology of each outbreak strain was 95.3% and the amino acid homology was 94.0%. The nucleotide and amino acid homology of outbreak strain and sporadic strain were 95.3% and 94.0%, respectively. The nucleotide homology and amino acid homology were 92.0 and 95.3, respectively. The phylogenetic tree analysis indicated that the distance between the outbreak of hepatitis E virus strain and the hepatitis E virus gene type 鈪，

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