ApoE基因多态性及累计飞行时间与飞行人员血脂水平关系的研究

发布时间：2018-08-11 15:20

【摘要】：目的研究ApoE基因多态性及累计飞行时间对飞行人员血脂水平的影响。方法以聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)法测定ApoE基因多态性。常规试剂盒测定血脂水平,包括血浆总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)。飞行人员个人信息采用问卷调查。结果飞行人员的血脂异常患病率为49.2%,高于国内普通人群的26.9%。飞行人员中ApoE等位基因ε2的频率高于国内一般人群,而ε4的频率低于一般人群,ApoE基因多态性与TC、LDL-C、ApoB的水平相关,而和TG、HDL-C的水平关系不明显。在高TC和高LDL-C血脂异常组,等位基因ε3的频率高于TC和LDL-C正常组,而等位基因ε2的情况刚好相反。累计飞行时间在15000h以上的飞行人员与累计飞行时间在10000h以下的人员相比,TC、HDL-C均明显增高(P0.05),而TG、LDL-C亦有增高趋势,但未有统计学差异。Logistic回归分析提示,BMI和血脂异常的发生相关,而累计飞行时间与体重指数(BMI)相关,抽烟、运动、喝酒等因素未发现与血脂异常及BMI有关。结论累计飞行时间是影响血脂水平的重要因素,累计飞行时间可以直接或间接地通过影响BMI而影响血脂水平。
[Abstract]:Objective to study the effect of ApoE gene polymorphism and cumulative flight time on lipid level of pilots. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to determine the polymorphism of ApoE gene. Blood lipid levels were measured by routine kit, including plasma total cholesterol, (TC), high density lipoprotein cholesterol (HDL-C), triglyceride (TG) and low density lipoprotein cholesterol (LDL-C). The personal information of pilots was investigated by questionnaire. Results the prevalence rate of dyslipidemia in pilots was 49.2%, which was higher than that of the general population in China (26.9%). The frequency of ApoE allele 蔚 2 was higher than that of the general population in China, and the frequency of 蔚 4 was lower than that of the normal population. The frequency of 蔚 4 allele was correlated with the level of TCU LDL-C ApoB, but not with the level of TGN HDL-C. The frequency of allele 蔚 3 in high TC and high LDL-C dyslipidemia group was higher than that in TC and LDL-C normal group, but the allele 蔚 2 was the opposite. Compared with those with cumulative flight time of less than 10, 000 hours, HDL-C of TCU increased significantly (P0.05), while LDL-C of TGN also tended to increase, but there was no significant correlation between BMI and dyslipidemia by logistic regression analysis. The cumulative flight time was correlated with body mass index (BMI), smoking, exercise, drinking and so on. There was no association with abnormal blood lipids and BMI. Conclusion cumulative flight time is an important factor affecting blood lipid level. Cumulative flight time can directly or indirectly affect blood lipid level by affecting BMI.
【作者单位】：中国民航大学航空医学研究所;东航西安分公司航医室;南航新疆分公司航医室;
【基金】：国家自然科学基金资助项目(30370358;60672175)
【分类号】：R856

【参考文献】