1型嗜肺军团菌的光纤倏逝波生物传感器快速测定法
发布时间：2018-08-26 19:48
【摘要】：目的利用光纤倏逝波生物传感器适合现场快速检测的优势,初步建立1型嗜肺军团菌的快速检测平台。方法优化最佳检测条件,并在此条件下确定检测Lp1的灵敏度和特异性,同时通过检测人工模拟样品确认该方法检测实际样本的灵敏度和特异性。结果该方法 40 min之内自动完成检测。捕获抗体与目标物最佳反应时间、反应体系与检测抗体的最佳反应时间均为10 min,捕获抗体最佳使用浓度是100μg/ml,检测抗体最佳使用浓度为20μg/ml。在此条件下得到该方法检测Lp1的灵敏度为1.8×103cfu/ml,检测的特异性强。结论光纤倏逝波生物传感器快速检测方法为大样本量Lp1污染严重水样的现场快速筛查提供一种新途径。
[Abstract]:Objective to establish a platform for rapid detection of Legionella pneumophila type 1 by using optical fiber evanescent wave biosensor. Methods the optimal detection conditions were optimized, and the sensitivity and specificity of Lp1 detection were determined under these conditions, and the sensitivity and specificity of the method were confirmed by detecting artificial simulated samples. Results the detection was completed automatically within 40 min of the method. The optimal reaction time for the capture antibody and the target, the optimal reaction time for the reaction system and the detection of the antibody were both 10 min,. The optimal concentration of the capture antibody was 100 渭 g / ml, and the best concentration for detection of the antibody was 20 渭 g / ml. Under these conditions, the sensitivity of this method for detecting Lp1 is 1.8 脳 10 ~ 3 cfur / ml, and the detection specificity is strong. Conclusion the fast detection method of fiber optic evanescent wave biosensor provides a new way for rapid screening of large sample size Lp1 polluted water samples.
【作者单位】： 中国疾病预防控制中心环境与健康相关产品安全所环境微生物室;
【分类号】：R82
[Abstract]:Objective to establish a platform for rapid detection of Legionella pneumophila type 1 by using optical fiber evanescent wave biosensor. Methods the optimal detection conditions were optimized, and the sensitivity and specificity of Lp1 detection were determined under these conditions, and the sensitivity and specificity of the method were confirmed by detecting artificial simulated samples. Results the detection was completed automatically within 40 min of the method. The optimal reaction time for the capture antibody and the target, the optimal reaction time for the reaction system and the detection of the antibody were both 10 min,. The optimal concentration of the capture antibody was 100 渭 g / ml, and the best concentration for detection of the antibody was 20 渭 g / ml. Under these conditions, the sensitivity of this method for detecting Lp1 is 1.8 脳 10 ~ 3 cfur / ml, and the detection specificity is strong. Conclusion the fast detection method of fiber optic evanescent wave biosensor provides a new way for rapid screening of large sample size Lp1 polluted water samples.
【作者单位】： 中国疾病预防控制中心环境与健康相关产品安全所环境微生物室;
【分类号】：R82
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