正加速度重复暴露大鼠脑差异表达基因的筛选
发布时间：2018-08-26 20:06
【摘要】：目的 筛选大鼠经正加速度 (Gositiveacceleration ,+Gz)重复暴露后脑的差异表达基因 ,探讨 +Gz重复暴露致脑损伤的分子机制。方法 应用抑制性消减杂交技术 ,构建高消减效率的 +Gz重复暴露大鼠脑cDNA消减文库 ;用差异筛选技术筛选阳性克隆 ;用序列分析确定阳性克隆的性质 ;用RT -PCR证实阳性克隆的可靠性。结果 从消减库中获得70个阳性克隆 ;经差异筛选后 ,选取其中 10个阳性克隆 ,序列分析表明 ,7个克隆与已知基因高度同源 ,3个为新的候选基因。RT -PCR证实了差异表达基因在 +Gz重复暴露大鼠脑中高表达。结论 用抑制性消减杂交技术筛选发现的 3个新的cDNA可能在 +Gz脑损伤的病理过程中起重要作用
[Abstract]:Objective to screen differentially expressed genes in rat brain after repeated exposure to positive acceleration (Gositiveacceleration, Gz), and to explore the molecular mechanism of brain injury induced by repeated Gz exposure. Methods the suppression subtractive hybridization technique was used to construct the cDNA subtractive library with high subtractive efficiency for repeated exposure of Gz in rat brain, the positive clones were screened by differential screening technique, the properties of the positive clones were determined by sequence analysis. The reliability of positive clones was confirmed by RT-PCR. Results 70 positive clones were obtained from subtractive library and 10 of them were selected after differential screening. Sequence analysis showed that 7 clones were highly homologous to the known genes, and 3 were new candidate genes. RT-PCR confirmed the overexpression of differentially expressed genes in the brain of rats exposed to repeated Gz exposure. Conclusion three new cDNA detected by suppression subtractive hybridization may play an important role in the pathological process of Gz brain injury.
【作者单位】： 第三军医大学附属西南医院病理学研究所 空军总医院临床分子生物学研究中心 第三军医大学附属西南医院病理学研究所 航空病研究中心 空军总医院临床分子生物学研究中心
【基金】：空军后勤部科研基金资助项目 (KH99190 76 )
【分类号】：R82
[Abstract]:Objective to screen differentially expressed genes in rat brain after repeated exposure to positive acceleration (Gositiveacceleration, Gz), and to explore the molecular mechanism of brain injury induced by repeated Gz exposure. Methods the suppression subtractive hybridization technique was used to construct the cDNA subtractive library with high subtractive efficiency for repeated exposure of Gz in rat brain, the positive clones were screened by differential screening technique, the properties of the positive clones were determined by sequence analysis. The reliability of positive clones was confirmed by RT-PCR. Results 70 positive clones were obtained from subtractive library and 10 of them were selected after differential screening. Sequence analysis showed that 7 clones were highly homologous to the known genes, and 3 were new candidate genes. RT-PCR confirmed the overexpression of differentially expressed genes in the brain of rats exposed to repeated Gz exposure. Conclusion three new cDNA detected by suppression subtractive hybridization may play an important role in the pathological process of Gz brain injury.
【作者单位】： 第三军医大学附属西南医院病理学研究所 空军总医院临床分子生物学研究中心 第三军医大学附属西南医院病理学研究所 航空病研究中心 空军总医院临床分子生物学研究中心
【基金】：空军后勤部科研基金资助项目 (KH99190 76 )
【分类号】：R82
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1 郝唯蔚,俞启福,赵云涛;飞行因素对人体血浆甘丙素的影响及其意义[J];海军总医院学报;2000年02期
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