舱室内爆炸对大鼠行为及海马NO、MDA、SOD的影响
发布时间：2018-09-01 21:17
【摘要】： 装甲车、坦克、舰船等带有舱室的兵器在现代战争中具有举足轻重的作用。舱室内爆炸对指战员的生理和心理造成了严重的影响,其伤型和严重程度都有别于其它爆炸伤。然而,以往研究大多局限于舱室内爆炸脏器损伤的特点,对舱室内爆炸致伤所引起的学习记忆和行为及其脑内氧自由基变化未见报道。因此,探讨舱室内爆炸的致伤生理和心理机制,对密闭舱室战创伤的救治,减少战时卫生减员,具有一定的军事理论价值和现实意义。 本研究通过按人鼠比例等比缩小模拟某型装甲运兵车舱室的密闭环境,采用纸质点爆源引爆,构建舱室内爆炸冲击伤应激大鼠模型,致伤后,观察大鼠不同时相点的学习记忆、行为变化,检测大鼠海马组织一氧化氮、丙二醛和超氧化物歧化酶含量动态变化。研究共分两部分进行:①探讨舱室内爆炸大鼠学习记忆及海马氧自由基的变化特点,建立舱室内爆炸冲击伤应激大鼠模型;②在成功建立动物模型的基础上,观察不同时相点舱室内爆炸和开阔地爆炸大鼠学习记忆、行为和海马内氧自由基变化的异同。研究结果如下: 1.初步建立了舱室内爆炸冲击伤应激大鼠模型,经旷场实验、Morris水迷宫实验等证实,该模型能较好的模拟舱室内爆炸应激反应的行为学变化特点,且致伤条件容易控制,复制简单,模型较稳定,为开展相关研究奠定了基础。 2.不同当量炸药舱室内爆炸后,600mg组、400mg组大鼠的Morris水迷宫逃避潜伏期、旷场水平运动和垂直运动得分较致伤前均显著增加(p0.01);200mg组大鼠水迷宫逃避潜伏期的变化与致伤前比较呈现出先低后高的现象,但无显著差异(p0.05),旷场水平运动和垂直运动得分较致伤前无显著差异(p0.05)。 3.不同当量炸药舱室内爆炸后,200mg组大鼠海马内SOD含量显著增高(p0.01),而NO、MDA含量变化并不显著(p0.05);600mg、400mg组大鼠海马内NO、MDA、SOD含量显著升高(p0.01),本研究结果舱室内爆炸大鼠海马内SOD的升高可能因应激状态下海马内NO、MDA等氧自由基产生过多而诱SOD的升高。 4.舱室内和开阔地相同当量炸药爆炸后,密闭组和开放组大鼠在旷场行为实验中水平运动和垂直运动的得分、迷宫潜伏期及穿梭箱平均反应时间均显著高于对照组(p0.01)。致伤后,舱内外大鼠学习记忆能力、行为改变的差异,主要集中在致伤后12小时至3天之间,在致伤后24小时差异最显著。旷场行为实验,密闭组的水平得分和垂直得分炸后前3天显著高于开放组;Morris水迷宫逃避潜伏期时长,炸后12小时至3天密闭组较开放组显著增加(p0.01);穿梭箱实验,密闭组平均反应时在炸后24小时到3天较开放组显著延长(p0.01)。 5.舱室内和开阔地相同当量炸药爆炸后,密闭组与开放组大鼠海马氧自由基动态变化趋势相同,NO、MDA较对照组均在致伤后6小时始显著增加(p0.05),24小时达高峰(p0.01);SOD在致伤后1小时较对照组出现显著差异(p0.01),6小时达高峰后快速降低。致伤后,密闭组与开放组之间氧自由基含量差异显著:伤后12小时、3天密闭组NO含量显著高于开放组(p0.01),伤后12小时密闭组SOD显著高于开放组(p0.01),MDA变化也不同,但无统计学意义(p0.05)。 总之,舱室内爆炸可以影响大鼠的学习记忆能力及海马氧自由基含量,不同当量炸药对大鼠学习记忆能力及其海马氧自由基含量影响不同。200mg的炸药可以改善大鼠的学习记忆能力,减轻氧自由基堆积对机体的损害,400mg、600mg炸药的爆炸结果则相反。舱室内和开阔地相同当量炸药爆炸后不同时相点,大鼠的学习记忆能力及海马氧自由基较对照组均发生明显的变化;相比舱室内和开阔地爆炸对大鼠学习记忆、行为改变以及氧自由基变化的程度以舱室内为重,主要表现在致伤后12小时至3天之间,尤以伤后24小时这一时段最为严重。这一结论对舱室内战伤的救治以及救治介入时机的选择具有重要意义。
[Abstract]:Armored vehicles, tanks, ships and other weapons with compartments play an important role in modern warfare. Indoor explosion has a serious impact on the physiology and psychology of the fighters. Its injury type and severity are different from other explosive injuries. The changes of learning memory and behavior and oxygen free radicals in brain caused by explosion injury have not been reported. Therefore, exploring the physiological and psychological mechanism of explosion injury in the cabin has certain military theoretical value and practical significance for the treatment of war wounds in airtight cabin and the reduction of health personnel in wartime.
In this study, a rat model of explosive blast injury was constructed by simulating the closed-loop environment of an armored personnel carrier cabin in the same ratio of human to rat. After injury, the learning and memory of rats were observed at different time points, and the behavior changes were detected. Nitric oxide, malondialdehyde and superoxide dismutase (SOD) in hippocampus were detected. The study was divided into two parts: (1) To investigate the changes of learning and memory and hippocampal oxygen free radicals in rats exposed to cabin explosion, and to establish a stress rat model of cabin explosion injury; (2) To observe the learning and memory of rats exposed to cabin explosion and open explosion at different time points on the basis of successful establishment of animal models. The similarities and differences between behavior and oxygen free radicals in hippocampus were studied.
1. The rat model of explosive blast injury in cabin was established. The open field experiment and Morris water maze experiment showed that the model could simulate the behavioral changes of explosive stress in cabin well, and the injury conditions were easy to control, the replication was simple and the model was stable, which laid a foundation for the related research.
2. After explosion in different equivalent explosive chamber, the escape latency, open field horizontal and vertical movement scores of Morris water maze in 600 mg group and 400 mg group were significantly higher than those before injury (p0.01); the escape latency of water maze in 200 mg group was lower than that before injury, but there was no significant difference (p0.05). The score of horizontal movement and vertical movement was not significantly different from that before injury (P0.05).
3. After explosion in different equivalent explosive chamber, SOD content in hippocampus of rats in 200 mg group increased significantly (p0.01), but NO and MDA content did not change significantly (p0.05); NO, MDA and SOD content in hippocampus of rats in 600 mg and 400 mg group increased significantly (p0.01). The results showed that the increase of SOD in hippocampus of rats exposed to explosive chamber might be due to oxygen such as NO and MDA in hippocampus under stress. The excessive production of free radicals induces the increase of SOD.
4. The scores of horizontal and vertical movement, the latency of maze and the average reaction time of shuttle box in the closed and open groups were significantly higher than those in the control group (p0.01). After the explosion, the differences of learning and memory abilities and behavior changes were mainly concentrated in the rats inside and outside the cabin. In open-field behavior test, the horizontal and vertical scores in the closed group were significantly higher than those in the open group at the first three days after explosion; Morris water maze escape latency was longer than that in the open group at 12 hours to 3 days after explosion; shuttle box test showed that the average reaction time in the closed group was significantly longer than that in the open group at 12 hours to 3 days after explosion (p0.01). The duration from 24 hours to 3 days after the blast was significantly longer than that in the open group (P0.01).
5. After the explosion of the same equivalent explosive in the cabin and open space, the dynamic changes of oxygen free radicals in the hippocampus of rats in the closed group and the open group were the same. NO and MDA increased significantly at 6 hours after injury (p0.05) and reached the peak at 24 hours (p0.01); SOD decreased significantly at 1 hour after injury (p0.01) and at 6 hours after the peak. After injury, the content of oxygen free radicals was significantly different between the closed group and the open group. The content of NO in the closed group was significantly higher than that in the open group at 12 hours and 3 days after injury (p0.01). SOD in the closed group was significantly higher than that in the open group at 12 hours after injury (p0.01). The change of MDA was not statistically significant (p0.05).
In a word, compartment explosion can affect the learning and memory ability of rats and the content of oxygen free radicals in hippocampus. Different equivalent explosives have different effects on the learning and memory ability of rats and the content of oxygen free radicals in hippocampus. Compared with the control group, the learning and memory abilities and oxygen free radicals in the hippocampus of rats were significantly changed at different time points after the explosion of the same equivalent explosive in the cabin and open space. From 12 hours to 3 days after injury, especially 24 hours after injury, this conclusion is of great significance to the treatment of war wounds in cabins and the timing of intervention.
【学位授予单位】：第三军医大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R82
[Abstract]:Armored vehicles, tanks, ships and other weapons with compartments play an important role in modern warfare. Indoor explosion has a serious impact on the physiology and psychology of the fighters. Its injury type and severity are different from other explosive injuries. The changes of learning memory and behavior and oxygen free radicals in brain caused by explosion injury have not been reported. Therefore, exploring the physiological and psychological mechanism of explosion injury in the cabin has certain military theoretical value and practical significance for the treatment of war wounds in airtight cabin and the reduction of health personnel in wartime.
In this study, a rat model of explosive blast injury was constructed by simulating the closed-loop environment of an armored personnel carrier cabin in the same ratio of human to rat. After injury, the learning and memory of rats were observed at different time points, and the behavior changes were detected. Nitric oxide, malondialdehyde and superoxide dismutase (SOD) in hippocampus were detected. The study was divided into two parts: (1) To investigate the changes of learning and memory and hippocampal oxygen free radicals in rats exposed to cabin explosion, and to establish a stress rat model of cabin explosion injury; (2) To observe the learning and memory of rats exposed to cabin explosion and open explosion at different time points on the basis of successful establishment of animal models. The similarities and differences between behavior and oxygen free radicals in hippocampus were studied.
1. The rat model of explosive blast injury in cabin was established. The open field experiment and Morris water maze experiment showed that the model could simulate the behavioral changes of explosive stress in cabin well, and the injury conditions were easy to control, the replication was simple and the model was stable, which laid a foundation for the related research.
2. After explosion in different equivalent explosive chamber, the escape latency, open field horizontal and vertical movement scores of Morris water maze in 600 mg group and 400 mg group were significantly higher than those before injury (p0.01); the escape latency of water maze in 200 mg group was lower than that before injury, but there was no significant difference (p0.05). The score of horizontal movement and vertical movement was not significantly different from that before injury (P0.05).
3. After explosion in different equivalent explosive chamber, SOD content in hippocampus of rats in 200 mg group increased significantly (p0.01), but NO and MDA content did not change significantly (p0.05); NO, MDA and SOD content in hippocampus of rats in 600 mg and 400 mg group increased significantly (p0.01). The results showed that the increase of SOD in hippocampus of rats exposed to explosive chamber might be due to oxygen such as NO and MDA in hippocampus under stress. The excessive production of free radicals induces the increase of SOD.
4. The scores of horizontal and vertical movement, the latency of maze and the average reaction time of shuttle box in the closed and open groups were significantly higher than those in the control group (p0.01). After the explosion, the differences of learning and memory abilities and behavior changes were mainly concentrated in the rats inside and outside the cabin. In open-field behavior test, the horizontal and vertical scores in the closed group were significantly higher than those in the open group at the first three days after explosion; Morris water maze escape latency was longer than that in the open group at 12 hours to 3 days after explosion; shuttle box test showed that the average reaction time in the closed group was significantly longer than that in the open group at 12 hours to 3 days after explosion (p0.01). The duration from 24 hours to 3 days after the blast was significantly longer than that in the open group (P0.01).
5. After the explosion of the same equivalent explosive in the cabin and open space, the dynamic changes of oxygen free radicals in the hippocampus of rats in the closed group and the open group were the same. NO and MDA increased significantly at 6 hours after injury (p0.05) and reached the peak at 24 hours (p0.01); SOD decreased significantly at 1 hour after injury (p0.01) and at 6 hours after the peak. After injury, the content of oxygen free radicals was significantly different between the closed group and the open group. The content of NO in the closed group was significantly higher than that in the open group at 12 hours and 3 days after injury (p0.01). SOD in the closed group was significantly higher than that in the open group at 12 hours after injury (p0.01). The change of MDA was not statistically significant (p0.05).
In a word, compartment explosion can affect the learning and memory ability of rats and the content of oxygen free radicals in hippocampus. Different equivalent explosives have different effects on the learning and memory ability of rats and the content of oxygen free radicals in hippocampus. Compared with the control group, the learning and memory abilities and oxygen free radicals in the hippocampus of rats were significantly changed at different time points after the explosion of the same equivalent explosive in the cabin and open space. From 12 hours to 3 days after injury, especially 24 hours after injury, this conclusion is of great significance to the treatment of war wounds in cabins and the timing of intervention.
【学位授予单位】：第三军医大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R82
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