模拟微重力效应下大鼠不同部位脑组织的氧化应激

发布时间：2018-09-05 08:01

【摘要】：本研究旨在观察不同时程模拟微重力效应下大鼠脑组织氧化应激的变化,为揭示模拟微重力效应下神经系统氧化应激的发生机制和开发相应的药物防护提供理论基础。将40只成年雄性Sprague-Dawley(SD)大鼠按体重配对原则随机分为对照组及不同时间(分别处理7,14,21,28d)尾悬吊模拟微重力效应组,应用Griess法、硫代巴比妥酸反应物法(thiobarbituric acid reactive substance assay,TBARS)、ELISA法和铁离子还原法(ferric reducing ability of plasma,FRAP)分别检测大鼠小脑、大脑皮层和海马组织内的活性氮(reactive nitrogen species,RNS)、丙二醛(malondialdehyde,MDA)、硝基酪氨酸(nitrotyrosine,NT)和总抗氧化能力(total antioxidant capacity,TAC)。结果显示,与对照组相比:(1)小脑组织在尾悬吊7d后NT含量明显升高,之后降低,28d再次升高;14d后MDA含量明显增加;21d后RNS含量显著升高,TAC显著降低;(2)大脑皮层在尾悬吊14d后NT含量显著升高;21d后MDA含量明显升高,TAC明显降低;(3)海马组织在尾悬吊7d后RNS含量明显升高,之后降低,28d后再次升高;21d后MDA含量明显升高;28d后NT含量显著上升;7d后TAC明显升高,持续至14d,然后恢复。上述结果表明,模拟微重力效应使得大鼠脑组织发生了氧化应激,不同部位脑组织对氧化应激的反应程度不同;在对不同时程模拟微重力效应的响应过程中,大鼠脑组织呈现了从适应性反应到不可逆性损伤的变化历程。
[Abstract]:The purpose of this study was to observe the changes of oxidative stress in rat brain under simulated microgravity effect at different time intervals, and to provide a theoretical basis for revealing the mechanism of oxidative stress in nervous system under simulated microgravity effect and for developing corresponding drug protection. Forty adult male Sprague-Dawley (SD) rats were randomly divided into two groups according to the principle of body weight pairing: the control group and the tail suspension simulated microgravity effect group (treated with 714 and 21 days for 28 days, respectively). Griess method was used. The active nitrogen (reactive nitrogen species,RNS), malondialdehyde (malondialdehyde,MDA), nitrotyrosine (nitrotyrosine,NT) and total antioxidant capacity (total antioxidant capacity,TAC) in cerebellum, cerebral cortex and hippocampus of rats were detected by thiobarbituric acid (thiobarbituric acid reactive substance assay,TBARS) Elisa and iron ion reduction assay (ferric reducing ability of plasma,FRAP), respectively. The results showed that: (1) NT content in cerebellar tissue increased significantly after 7 days of tail suspension. (2) the content of NT in cerebral cortex increased significantly after 14 days of tail suspension. After 21 days of suspension, the content of MDA increased significantly. (3) the content of MDA in hippocampus decreased significantly after 14 days of tail suspension. (3) the content of NT in hippocampus increased significantly after 14 days of tail suspension. (3) the content of MDA in hippocampus increased significantly after 14 days of tail suspension. (3) the content of MDA in hippocampus increased significantly after 14 days of suspension. The content of RNS increased significantly after 7 days of tail suspension. The content of MDA increased significantly after 28 days and then increased again after 21 days. After 28 days, the content of NT increased significantly. After 7 days, the content of TAC increased significantly, which lasted until 14 days, and then recovered. The results indicated that the simulated microgravity effect caused oxidative stress in rat brain, and the reaction degree of different brain tissues to oxidative stress was different, and in the course of response to simulated microgravity effect at different time intervals, The brain tissue of rats showed a process from adaptive response to irreversible injury.
【作者单位】：中国航天员科研训练中心;河北省望都县医院;
【基金】：supported by the National Natural Science Foundation of China (No. 30570452, 30600759)
【分类号】：R855

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