模拟晕船大鼠晕船行为学指标的筛选及适应规律的观察

发布时间：2018-09-05 21:06

【摘要】：目的:筛选大鼠晕船行为学指标,探讨大鼠晕船适应时间及规律。方法:运用Crampton旋转刺激模型,对SD大鼠进行重复刺激,实时观察晕船刺激过程中尿液、粪便排泄量及刺激后自发活动的变化;采用抗晕药物干预法检验各行为学指标的特异性。结果:与正常对照组相比,晕船刺激第1天刺激组大鼠粪便量显著增加(P0.05),自发活动显著减少(P0.01);随着刺激天数的增加,刺激组粪便量、自发活动均逐渐恢复,且与刺激天数间存在一定相关性(P0.01),并分别于第9天和第7天下降至正常对照水平;用药组以东莨菪碱(0.5、1mg/kg)灌胃后再进行旋转刺激,各行为学指标与正常对照组相比均无统计学差异。结论:旋转刺激时的排便量和刺激后的自发活动量可有效判断大鼠晕动病,连续刺激9d左右后大鼠可达到晕船适应。
[Abstract]:Aim: to study the adaptation time and regularity of seasickness in rats by screening the behavioral indexes of seasickness in rats. Methods: Crampton rotation stimulation model was used to observe the changes of urine, fecal excretion and spontaneous activity during seasickness stimulation in SD rats, and the specificity of each behavioral index was tested by anti-halo drug intervention. Results: compared with the normal control group, the fecal quantity and spontaneous activity in the seasickness stimulation group increased significantly on the first day after seasickness stimulation (P0.05) and the spontaneous activity decreased significantly (P0.01), and with the increase of stimulation days, the fecal quantity and spontaneous activity gradually recovered in the stimulation group. There was a certain correlation with the days of stimulation (P0.01), and it was decreased to the normal control level on the 9th and 7th day, the rats in the treated group were treated with scopolamine (0.5 mg / kg) and then rotated stimulation, and the control group was fed with scopolamine (0.5 mg / kg), and then treated with scopolamine (0.5 mg / kg). There was no statistical difference between the behavioral indexes and the normal control group. Conclusion: the amount of defecation and the amount of spontaneous activity after rotation stimulation can effectively judge the motion sickness of rats. After 9 days of continuous stimulation, the rats can achieve seasickness adaptation.
【作者单位】：第二军医大学海军医学系军队卫生学教研室;
【分类号】：R835.2

【共引文献】