海水淹溺型急性肺损伤兔血管内皮生长因子及其受体的表达

发布时间：2018-10-29 15:06

【摘要】：目的探讨海水淹溺型急性肺损伤(SWD-ALI)后血清及组织血管内皮生长因子(VEGF)及其可溶性受体-1(sVEGFR-1)的表达变化及意义。方法健康成年新西兰兔28只,采用随机数字法均分为对照组和模型组(制作SWD-ALI模型),分别用于3h和6h时间点(每组7只)的观察。分别检测各组动物的肺微血管通透指数、肺湿/干重比和肺组织病理学评分;ELISA法检测血清、肺泡灌洗液(BALF)及肺组织的VEGF及sVEGFR-1表达,RTPCR法检测肺组织mRNA表达;免疫组化法检测VEGF在肺组织的分布。结果海水淹溺后,肺组织湿/干重比、肺微血管通透指数均增高,肺组织病理损伤重,6h时点肺组织湿/干重比略低于3h,而肺组织病理学积分仍高于3h。血清中VEGF和sVEGFR-1水平均明显升高,并与损伤时间(r=0.837,r=0.761,P0.01)和病理积分呈正相关(r=0.796,r=0.839,P0.01)。VEGF和sVEGFR-1在肺组织及BALF中的表达均明显减低,两者mRNA的表达也明显减低,6h时有所回升。免疫组化提示VEGF在对照组大量表达,分布均匀,淹溺后表达明显减少,分布不均。结论SWD-ALI时,VEGF和sVEGFR-1表达异常,两者变化规律一致,其水平与肺损伤的程度相关。
[Abstract]:Objective to investigate the expression of vascular endothelial growth factor (VEGF) and soluble receptor-1 (sVEGFR-1) in serum and tissues after seawater drowning acute lung injury (SWD-ALI). Methods Twenty-eight healthy New Zealand rabbits were randomly divided into two groups: control group and model group (SWD-ALI model), which were observed at 3h and 6h time points (7 rabbits in each group), respectively. The pulmonary microvessel permeability index, lung wet / dry weight ratio and lung histopathological score were measured, the expression of VEGF and sVEGFR-1 in serum, alveolar lavage fluid (BALF) and lung tissue was detected by ELISA method, and the expression of mRNA in lung tissue was detected by RTPCR method. The distribution of VEGF in lung tissue was detected by immunohistochemical method. Results after seawater drowning, the wet / dry weight ratio of lung tissue, the permeability index of pulmonary microvessel increased, and the lung tissue pathological injury was serious. At 6 h, the wet / dry weight ratio of lung tissue was lower than 3 h, but the pathological score of lung tissue was still higher than 3 h. The levels of VEGF and sVEGFR-1 in serum increased significantly, and were positively correlated with the injury time (r = 0.837) and the pathological score (r = 0.796n / r = 0.839), and a positive correlation was found between the time of injury (r = 0.837) and the pathological score (P < 0.01). The expression of P0.01). VEGF and sVEGFR-1 in lung tissue and BALF was significantly decreased, and the expression of mRNA was also decreased, and then increased at 6 h. Immunohistochemistry showed that the expression of VEGF in the control group was large and evenly distributed, but after drowning, the expression of VEGF was significantly decreased and unevenly distributed. Conclusion the expression of VEGF and sVEGFR-1 in SWD-ALI is abnormal, and the changes of VEGF and sVEGFR-1 are consistent. The level of VEGF and sVEGFR-1 is related to the degree of lung injury.
【作者单位】：海军总医院呼吸内科;海军总医院干一科;
【基金】：全军“十一五”后勤科研计划课题(06-3305)
【分类号】：R83;R563.8

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