慢性低频电刺激对模拟失重后肌萎缩及肌梭传入放电的影响

发布时间：2018-11-04 16:58

【摘要】：目的　观察在吊尾的不同时期 (3、7、14d)及吊尾加慢性低频电刺激 (CLFS) 14d后比目鱼肌肌梭的传入放电有何改变。方法　采用电生理技术 ,在大鼠脊髓背根分离神经细束 ,记录吊尾不同时期比目鱼肌在动静式(ramp and hold)牵拉过程中肌梭的传入放电 ,以及吊尾加CLFS 14d后肌梭传入放电的变化。结果　①模拟失重14d后 ,比目鱼肌发生明显的萎缩 ,比目鱼肌湿重体重比由吊尾前的 38.8(mg·10 0 g- 1 )降低到 2 3.1,而吊尾加CLFS 14d后比目鱼肌湿重体重比仅下降到 32 .5 ,表明CLFS可有效的防止模拟失重所造成的肌萎缩。②模拟失重 14d后 ,比目鱼肌肌梭的传入放电比正常对照组明显减少 ,而吊尾加CLFS 14d后 ,肌梭传入放电的各项指标均有不同程度的升高 ,与单纯吊尾组相比 ,有显著性差异 (P 0 .0 5 )。结论　本研究结果提示模拟失重可引起肌肉萎缩及肌梭的传入放电减少 ,而慢性低频电刺激可以部分地对抗这些变化。
[Abstract]:Objective to observe the changes of afferent discharge in soleus muscle spindle after 14 days of tail suspension and chronic low frequency electrical stimulation of (CLFS). Methods the fine fascicles of the nerve were isolated from the dorsal root of the spinal cord of rats by electrophysiological technique. The afferent discharge of the muscle spindle was recorded during the traction of the soleus muscle in the different period of tail suspension. And the changes of afferent discharge of muscle spindle after 14 days of tail suspension and CLFS. Results (1) after 14 days of simulated weightlessness, the soleus muscle shrank obviously, and the wet weight / body weight ratio of soleus muscle decreased from 38.8 (mg 100 g -1) before tail suspension to 23.1%. However, the wet weight / body weight ratio of soleus muscle decreased to only 32.5 after 14 days of suspension of tail and CLFS, indicating that CLFS could effectively prevent muscle atrophy caused by simulated weightlessness. 2 after 14 days of simulated weightlessness, the afferent discharge of muscle spindle of soleus muscle decreased significantly compared with that of normal control group. However, the indexes of muscle spindle afferent discharge increased in different degree after 14 days of tail suspension plus CLFS, and there was significant difference compared with the single tail suspension group (P 0.05). Conclusion the results suggest that simulated weightlessness can induce muscle atrophy and decrease the afferent discharge of muscle spindle, but chronic low frequency electrical stimulation can partly counteract these changes.
【作者单位】：中山大学中山医学院生理学教研室西安交通大学医学院生理学教研室西安市莲湖区卫生局
【基金】：陕西省自然科学基金资助 (No .98SM5 5 )
【分类号】：R852.22

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