局部氧气干预对高温复合创伤大鼠创面愈合的影响

发布时间：2018-11-20 07:27

【摘要】：目的:通过建立高温高湿环境下大鼠腹部创伤动物模型,用局部氧气干预创面的方法,探讨这一特殊环境下创面组织的病理形态学变化以及局部氧气干预对创面愈合的影响,为临床伤口护理提供理论依据。方法:将24只创伤大鼠随机分为常温对照组(NEC)、常温氧疗组(NEO)、高温对照组(HHE)、高温氧疗组(HHO)4组,每组6只。在相应干预后的不同时间对各组动物的平均动脉压(MAP)、创面愈合时间、细菌学定量、病理形态学进行观察和检测。结果:HHE组较NEC组创面肿胀程度严重,创面愈合时间长,HHO组较HHE组创面肿胀程度轻,创面愈合时间短。光镜和电镜观察到HHE组创面肌肉纤维的病理变化均较NEC组显著,且损伤呈进行性加重,HHO组肌肉组织损伤程度较HHE组轻。细菌学定量检测HHE组创面细菌数在同一时相比NEC组高,且随时间的延长呈显著增长趋势,HHO组创面细菌数量与HHE组比较差异有显著性意义(P0.05)。结论:高温高湿环境下创面组织病理损伤严重,细菌繁殖快,感染时间提前且严重,创面愈合速度慢;局部氧气干预可抑制创面组织细菌繁殖,对大鼠腹部刀割创面有明显的促修复作用。
[Abstract]:Objective: to investigate the pathomorphological changes of wound tissue and the effect of local oxygen intervention on wound healing by establishing an animal model of abdominal trauma in rats under high temperature and high humidity. To provide theoretical basis for clinical wound nursing. Methods: 24 traumatized rats were randomly divided into normal temperature control group (NEC), (NEO), hyperthermia control group, (HHE), hyperthermia oxygen therapy group (HHO) 4), 6 rats in each group. The wound healing time, bacteriological quantity and pathomorphology of (MAP), wound were observed and detected at different time after intervention. Results: the degree of wound swelling in HHE group was more serious than that in NEC group, and the wound healing time was longer. In HHO group, the degree of wound swelling was lighter and the healing time was shorter than that in HHE group. The pathological changes of muscle fibers in HHE group were significantly higher than those in NEC group, and the injury was progressive. The degree of muscle tissue injury in HHO group was lighter than that in HHE group. The number of bacteria in wound of HHE group was higher than that of NEC group at the same time, and the number of bacteria in HHO group was significantly higher than that in HHE group (P0.05). Conclusion: under high temperature and high humidity environment, the wound tissue has serious pathological damage, the bacteria propagate fast, the infection time is early and serious, and the wound healing speed is slow. Local oxygen intervention could inhibit the propagation of bacteria in wound tissue and could promote the repair of abdominal incision wound in rats.
【作者单位】：解放军第一军医大学南方医院护理部解放军第一军医大学南方医院护理部解放军第一军医大学高温医学研究室解放军第一军医大学高温医学研究室解放军第一军医大学病理教研室
【基金】：全军医学科研基金“十五”计划资助(01MAI33)~~
【分类号】：R82

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