高正加速度环境对猴翼外肌c-jun表达的影响

发布时间：2018-11-20 18:58

【摘要】：目的:观察高正加速度环境对猴翼外肌组织病理学及c-jun表达的影响。方法:实验于2005-12/2006-12在解放军第三○六医院和航天医学工程研究所完成。①实验动物:健康雄性恒河猴9只,随机数字表法分为4组:+1加速度300s对照组2只、+15加速度200s组2只、+18加速度165s组2只、+21加速度140s组3只。②实验方法:采用改造后的五八型动物离心机模拟飞船升空和返回时产生的超重,各组动物分别承受相应的胸-背向超重作用及持续时间。③实验评估:离心超重作用结束后,解剖取猴翼外肌组织,光学显微镜下观察翼外肌组织病理学变化。采用免疫组化PicTureTM两步法观察翼外肌细胞c-jun的表达,细胞核呈棕色为c-jun阳性反应。结果:9只恒河猴均进入结果分析。①+1加速度300s对照组翼外肌无明显变化,表现为正常的肌组织特征。其余3组翼外肌组织局部间质充血和出血。②+1加速度300s对照组翼外肌细胞核不着色或呈淡黄色,为c-jun阴性或弱阳性表达,细胞质着色不明显。其余3组翼外肌细胞核和细胞质均呈深棕色,为c-jun强阳性表达,3组间无明显差别。结论:超过+15加速度环境可引起猴翼外肌组织局部间质充血和出血,增强肌细胞c-jun基因的表达。
[Abstract]:Aim: to observe the effect of high positive acceleration environment on histopathology and c-jun expression in the lateral pterygoid muscle of monkey. Methods: the experiment was carried out in the sixth Hospital of PLA and the Institute of Aerospace Medical Engineering in December 2005 and December 2006. 1 Experimental animals: 9 healthy male rhesus monkeys were randomly divided into 4 groups: 1 acceleration 300 s control group 2; There were 2 rats in the 200s group with 15 acceleration, 2 rats in the 165s group with 18 acceleration, 3 rats in the 140s group of 21 acceleration. Experimental evaluation: after centrifugation, the outer pterygoid muscle tissue was dissected and the pathological changes of the lateral pterygoid muscle were observed under optical microscope. The expression of c-jun in outer pterygoid muscle cells was observed by immunohistochemical PicTureTM two-step method. The nucleus was brown positive for c-jun. Results: all the 9 rhesus monkeys entered the result analysis. The lateral pterygoid muscle showed no obvious change in the 300s acceleration control group, showing normal muscle tissue characteristics. In the other three groups, interstitial congestion and hemorrhage were observed in the other three groups. The nucleus of the outer pterygoid muscle was not stained or yellowish in the control group with 21 acceleration of 300s, but the expression of c-jun was negative or weak positive, but the cytoplasm staining was not obvious. The nuclei and cytoplasm of the outer pterygoid muscles in the other three groups were dark brown and strongly positive for c-jun. There was no significant difference among the three groups. Conclusion: over 15 acceleration environment can induce local interstitial congestion and hemorrhage in the lateral pterygoid muscle of monkey and enhance the expression of c-jun gene in muscle cells.
【作者单位】：解放军第三0六医院全军口腔疾病诊治中心解放军第三0六医院全军口腔疾病诊治中心解放军第三0六医院病理实验科解放军第三0六医院全军口腔疾病诊治中心解放军第三0六医院病理实验科
【基金】：国家自然科学基金资助(30672319) 全军“十一五”计划课题项目基金资助(06MA004)~~
【分类号】：R85

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