模拟失重下空间诱变菌感染巨噬细胞炎症相关microRNAs的筛选及生物信息学分析

发布时间：2018-12-13 03:34

【摘要】：目的探索模拟失重下空间诱变大肠杆菌(T1-13)感染巨噬细胞RAW264.7后差异表达的microRNAs(miRNAs)及其意义。方法采用第二代测序技术检测模拟失重染菌组与对照组巨噬细胞miRNAs差异表达,通过生物信息学预测差异miRNAs的靶基因,并对靶基因进行GO功能富集分析、KEGG信号通路分析以及STRING蛋白互作分析。结果模拟失重染菌组与对照组间筛选出33个差异miRNAs,下调23个,上调10个。这些miRNAs互补结合的靶基因参与了MAPK通路、WNT通路、轴突导向以及TGF-β等信号通路,其中关键下调的靶基因是MAPK9,MAPK14,MAP3K7,MAP3K14,PIK3CA,PIK3CB,PIK3CD,MYC,JUN,SMAD4;关键上调的靶基因是AKT3,MAPK8,MAPK9,MAPK10,MAP2K1,PIK3CD。结论模拟失重下空间诱变大肠杆菌感染巨噬细胞后miRNAs表达出现显著差异,这些miRNAs可能通过调控MAPK,WNT以及TGF-β等通路参与失重条件下炎症反应的发生。
[Abstract]:Objective to explore the differential expression of microRNAs (miRNAs) in macrophages infected with space mutated Escherichia coli (T1-13) under simulated weightlessness and its significance. Methods the second generation sequencing technique was used to detect the differential expression of miRNAs in macrophages of simulated weightlessness group and control group. The target genes of differential miRNAs were predicted by bioinformatics, and the GO function enrichment analysis was carried out on the target genes. KEGG signal pathway analysis and STRING protein interaction analysis. Results 33 differences in miRNAs, were down-regulated by 23 and increased by 10 between the simulated weightlessness group and the control group. These complementary miRNAs binding target genes are involved in MAPK pathway, WNT pathway, axon orientation and TGF- 尾 signaling pathway. The key down-regulated target gene is MAPK9,MAPK14,MAP3K7,MAP3K14,PIK3CA,PIK3CB,PIK3CD,MYC,JUN,SMAD4;. The key up-regulated target gene is AKT3,MAPK8,MAPK9,MAPK10,MAP2K1,PIK3CD. Conclusion there is a significant difference in miRNAs expression in macrophages infected by space mutagenesis under simulated weightlessness. These miRNAs may be involved in inflammatory reaction under weightlessness through regulating MAPK,WNT and TGF- 尾 pathways.
【作者单位】：石河子大学医学院;石河子大学第一附属医院检验科;锦州医科大学;中国航天员科研训练中心航天医学基础与应用国家重点实验室;解放军总医院南楼呼吸科;
【基金】：国家重点基础研究发展计划(2014CB744404)
【分类号】：R852.22

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