模拟失重对大鼠肺动脉eNOS表达的影响

发布时间：2018-12-14 22:41

【摘要】：目的通过研究模拟失重对大鼠肺动脉血管内皮型一氧化氮合酶(eNOS)表达的影响,探讨肺动脉反应性改变的发生机制。方法采用尾悬吊大鼠模拟失重,分为模拟失重组(尾悬吊14天)和对照组,每组8只健康雄性Wistar大鼠,用蛋白免疫印迹分析模拟失重组和对照组大鼠肺动脉组织eNOS表达,硝酸还原酶法测定肺动脉组织一氧化氮(NO)含量。结果同对照组相比,模拟失重组大鼠肺动脉组织eNOS表达增强,胶片图像扫描后的相对光密度值为247±2·60,对照组的相对光密度值为141±1·72,两组间有显著性差异(P0·01)。模拟失重组肺动脉组织NO含量亦增加,其浓度为15·7±4·1μmol/L,对照组为7·6±3·2μmol/L,两组间有显著性差异(P0·05)。结论模拟失重条件下肺动脉eNOS表达增加可能促进了立位耐力不良的发生。
[Abstract]:Objective to investigate the effect of simulated weightlessness on the expression of nitric oxide synthase (eNOS) in rat pulmonary artery endothelium and to explore the mechanism of pulmonary artery reactivity. Methods the rats with tail suspension were divided into simulated weightlessness group (tail suspension for 14 days) and control group. Eight healthy male Wistar rats were used in each group. Western blot analysis was used to simulate the loss of weight and the expression of eNOS in pulmonary artery of control group. The content of nitric oxide (NO) in pulmonary artery was determined by nitrate reductase method. Results compared with the control group, the expression of eNOS in the pulmonary artery of the simulated lost recombination rats was enhanced. The relative optical density of the two groups was 247 卤2.60 after film scanning and 141 卤1.72 in the control group. There was a significant difference between the two groups (P0.01). The content of NO in simulated lost pulmonary artery tissue was also increased, the concentration of which was 15 7 卤4 1 渭 mol/L, in the control group was 7 6 卤3 2 渭 mol/L, there was significant difference between the two groups (P0 05). Conclusion the increased expression of eNOS in pulmonary artery under simulated weightlessness may promote the occurrence of orthostatic dysphoria.
【作者单位】：
【基金】：武器装备预研基金项目(51400020104JB001) 全军医学科研“十五”基金重点项目(04Z020) 全军医学科研“十一五”计划专项课题(06Z048)
【分类号】：R852.22

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