5km高空缺氧对小鼠肝脾及血清中Zn,Cu和Fe的影响

发布时间：2019-02-19 13:44

【摘要】：目的:探讨5km高空缺氧和不缺氧对小鼠肝、脾及血清中Zn,Cu,Fe的影响.方法:高空缺氧试验:将雄性昆明种小鼠20只,随机分为实验组(5km高空缺氧30min组)和地面对照组,每组10只.将实验组小鼠置于低压舱内,以40m/s的速度上升到5km,停留30min后,以40m/s速度降至地面;地面对照组放置在另一低压舱中留在地面.连续7d后,抽取小鼠静脉血液并处死小鼠取肝、脾.高空不缺氧试验:分组及实验方法同上.舱内压力为54.02kPa,其中氧分压为21.2kPa.舱内容积百分比为41.8%氧和58.2%氮的混合气体.用测氧仪监测舱内氧压,保持其肺泡氧分压与地面相同.采用火焰原子吸收法测定血清Zn,Cu和Fe的含量.结果:5km高空缺氧组与对照组相比较,小鼠肝中Cu和Fe含量均显著降低,差异有统计学意义(P0.05);脾中Fe的含量明显升高,差异有统计学意义(P0.05);两组血清中Zn,Cu和Fe含量均无明显变化,差异无统计学意义(P0.05).5km高空不缺氧组与对照组相比较,小鼠肝、脾中Zn,Cu和Fe含量均降低,差异具有统计学意义(P0.05),血清中Zn,Cu和Fe含量均无明显变化,差异无统计学意义(P0.05).结论:5km高空缺氧时对小鼠肝中Cu和Fe的合成产生影响;5km高空缺氧和不缺氧时小鼠血清中Zn,Cu和Fe的含量无明显变化,表明其含量可能与机体缺氧无关.
[Abstract]:Aim: to investigate the effects of hypoxia and non-hypoxia of 5km on Zn,Cu,Fe in liver, spleen and serum of mice. Methods: twenty male Kunming mice were randomly divided into experimental group (5km 30min group) and ground control group (10 mice in each group). The mice in the experimental group were placed in a low pressure chamber, and the speed of 40m/s was increased to 5 km, then dropped to the ground with the speed of 40m/s after staying in 30min, while the ground control group was placed on the ground in another chamber. After 7 days, the blood was taken from the vein of the mice and the liver and spleen of the mice were killed. High altitude non-anoxic test: grouping and experimental methods are the same as above. The chamber pressure is 54.02 KPA, and the oxygen partial pressure is 21.2 KPA. Tank volume percentage of 41.8% oxygen and 58.2% nitrogen mixture. The oxygen pressure in the cabin is monitored by oxygen meter, and the alveolar oxygen pressure is the same as on the ground. The content of Zn,Cu and Fe in serum was determined by flame atomic absorption spectrometry. Results: compared with the control group, the content of Cu and Fe in the liver of 5km group was significantly lower than that in the control group (P0.05), and the content of Fe in spleen was significantly increased (P0.05). The contents of Zn,Cu and Fe in serum of the two groups had no significant change (P0.05). Compared with the control group, the contents of Zn,Cu and Fe in the liver and spleen of mice were decreased in the 5km high-altitude hypoxia group. The difference was statistically significant (P0.05), serum Zn,Cu and Fe levels were not significantly changed, the difference was not statistically significant (P0.05). Conclusion: 5km has an effect on the synthesis of Cu and Fe in liver of mice under high altitude hypoxia, and the contents of Zn,Cu and Fe in serum of mice under high altitude hypoxia and no hypoxia of 5km have no significant change, indicating that the contents of Zn,Cu and Fe may not be related to hypoxia.
【作者单位】：吉林医药学院国有资产与实验室管理处吉林医药学院营养学教研室吉林医药学院营养学教研室
【分类号】：R85

【共引文献】