华北地区军事坑道内储水真菌污染原因分析

发布时间：2019-07-14 19:03

【摘要】：目的:对华北地区29条军事坑道内32个平均储存20年左右的水库储水进行真菌学及其污染原因分析,为采取预防措施提供依据。方法:用沉降法对坑道空气真菌浓度进行检测。用萨布罗琼脂培养基以倾注法检测储水真菌浓度。对部分检出的空气和储水真菌菌株根据菌落形态特征及显微镜下生长结构进行鉴定。结果:①有散在或连成片状真菌斑膜的占37.5%,有大面积片状真菌斑膜的占25.0%,水面全部被真菌斑膜覆盖的占9.4%。②29条坑道空气真菌浓度平均2 532 CFU/m3。32个水库平均真菌浓度819.9 CFU/m l。③对1 734株空气真菌菌株及1 125株储水真菌菌株鉴定,均以青霉属(45%及46.8%)、曲霉属(12.9%及13.7%)、链格孢属(8.9%及6.8%)为优势菌。结论:储水真菌污染较普遍,污染原因主要是未采取消毒措施,空气中飘浮的真菌通过孔门及缝隙进入水库沉降水面造成污染。如果采取消毒措施,切断传播途径,严格管理制度,可以防止真菌污染,确保储水的卫生质量。
[Abstract]:Objective: to analyze the mycology and pollution causes of 32 reservoirs stored in 29 military tunnels in North China for an average of about 20 years, so as to provide the basis for taking preventive measures. Methods: the concentration of fungi in tunnel air was detected by sedimentation method. The concentration of water storage fungi was detected by pouring method in Sabro Agar medium. Some strains of air and water storage fungi were identified according to the morphological characteristics of colonies and the growth structure under microscope. Results: 137.5% of them were scattered or connected to flake fungal plaque, 25.0% of them had large area flake fungal plaque, and 9.4% of 229 tunnels were covered with fungal plaque. The average fungal concentration of 229 tunnel air fungi was 819.9 CFU/m / L ~ 3 against 1 734 strains of air fungi and 1 125 strains of water storage fungi, and the average concentration of fungi in 229 tunnels was 819.9 CFU/m / L ~ 3 against 1 734 strains of air fungi and 1 125 strains of water storage fungi. Penicillium (45% and 46.8%), Aspergillus (12.9% and 13.7%) and Alternaria (8.9% and 6.8%) were the dominant bacteria. Conclusion: fungal pollution in water storage is more common, and the main cause of pollution is that no disinfection measures have been taken. The floating fungi in the air enter the water surface of the reservoir through the hole door and gap, resulting in pollution. If disinfection measures are taken, transmission routes can be cut off and strict management system can be taken to prevent fungal pollution and ensure the hygienic quality of stored water.
【作者单位】：解放军第251医院;
【基金】：北京军区“十一五”计划课题(No.06BJ005)
【分类号】：R82

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