金属—有机骨架材料ZIF-8用于选择性萃取藏药材鬼箭锦鸡儿中活性成分

发布时间：2021-01-12 22:56

　　药用植物是人类获取临床药物的主要来源之一,其所含活性成分的研究是寻找先导化合物、开发新药的有效途径。如抗疟药物青蒿素即是从菊科蒿属植物黄花蒿中分离获取。然而,从基质复杂的药用植物中直接萃取分离目标成分具有挑战性。为此,人们先后将硅胶、氧化铝、大孔吸附树脂和活性炭等多孔材料应用于药用植物中目标成分的萃取分离。由于材料结构和表面性质等方面的原因,这些材料的分离选择性均受到不同程度的限制。因此,寻找和研制高选择性和较大吸附容量的多孔材料对于高效制备药用植物中的目标成分具有重要意义。金属-有机骨架材料（MOFs）是一类由金属离子或离子簇与有机功能配体自组装形成的多孔功能性材料,具有较大比表面积、良好热稳定性和化学稳定性、结构和孔可调等优点,目前在气体储存、催化、传感和分离等领域得到了广泛的研究和应用。此外,MOFs半有机骨架、规则有序的孔结构和活泼的金属配位点使其在选择性吸附萃取方面具有良好的应用潜力,其应用已涵盖从简单基质中的有机小分子到复杂基质中的多肽、蛋白质、染料等生物大分子。鉴于MOFs在复杂样品中具有良好的选择性吸附萃取能力,将MOFs用于选择性萃取药用植物中的活性成分将是一个很有意... 

【文章来源】：天津大学天津市 211工程院校 985工程院校 教育部直属院校

【文章页数】：92 页

【学位级别】：硕士

【文章目录】：
摘要
abstract
Chapter 1 Literature review
    1.1 Research progress of Caragana jubata （C. jubata）
        1.1.1 Chemical components
        1.1.2 Pharmacological activities
        1.1.3 Separation methods research
    1.2 Metal-Organic Frameworks
        1.2.1 Properties of MOFs
        1.2.2 Synthesis methods
        1.2.3 Applications
    1.3 Research topic
Chapter 2 MOFs screening and identification of main components of DCM extract
    2.1 Introduction
    2.2 Experimental section
        2.2.1 Chemicals and reagents
        2.2.2 Instruments
        2.2.3 MOFs screening
        2.2.4 Preparation of DCM extract of C. jubata
        2.2.5 Separation and identification
    2.3 Results and discussions
        2.3.1 MOFs screening
        2.3.2 Structure identification
Chapter 3 Selective extraction and adsorption mechanism of ZIF-8
    3.1 Experimental section
        3.1.1 Chemicals and reagents
        3.1.2 Instruments
        3.1.3 Synthesis and characterization of ZIF-8
        3.1.4 Extraction and condition optimization of ZIF-8 for compound 1
        3.1.5 Recovery test of compound 1
        3.1.6 Adsorption mechanism study
    3.2 Results and discussions
        3.2.1 Characterization of ZIF-8
        3.2.2 Selective extraction of compound 1
        3.2.3 Optimization of adsorption/desorption conditions
        3.2.4 Results of recovery test
        3.2.5 Adsorption mechanism
    3.3 Extraction of active components on other MOFs
Chapter 4 Pharmacological activities of the selectively adsorbed compound
    4.1 Experimental section
        4.1.1 Chemicals and reagents
        4.1.2 Instruments
    4.2 Antioxidant activity analysis
        4.2.1 Scavenging activity against DPPH free radical
        4.2.2 Assay of ferric reducing/antioxidant power （FRAP）
        4.2.3 Inhibitory effect on lipid peroxidation induced by Fe2+/ascorbate
    4.3 Antiplatelet aggregation activity in vitro
    4.4 Antiproliferative activity assay
    4.5 Results and discussions
        4.5.1 Antioxidant activity analysis
        4.5.2 Antiplatelet aggregation activity in vitro
        4.5.3 Antiproliferative activity assay
Chapter 5 Conclusions and prospects
    5.1 Conclusions
    5.2 Prospects
References
Appendices
Notes on publications and participation in scientific research
Acknowledgements


【参考文献】：
期刊论文
[1]鬼箭锦鸡儿抗血栓活性部位筛选[J]. 贺春荣,郭丽娜,张怡,沈丹,杨学东.  中国中药杂志. 2016(13)
[2]鬼箭锦鸡儿化学成分的研究[J]. 宋萍,李小娟,贾岩岩.  中成药. 2011(11)
[3]New Chemical Compound from Caragana jubata（pall.） Poir.[J]. SONG Ping~(1*),YANG Xin-zhou~2 and YU Jun~1 1.Department of Chemistry,Qinghai University for Nationalities,Xining 810007,P.R.China; 2.Department of Pharmaceutical Sciences,University of Basel,Basel CH-4056,Switzerland.  Chemical Research in Chinese Universities. 2010(04)
[4]鬼箭锦鸡儿中紫檀烷类化合物抗真菌活性研究(英文)[J]. 宋萍,杨新洲,于军.  中国现代应用药学. 2009(09)
[5]鬼箭锦鸡儿化学成分的研究[J]. 宋萍,杨赵立,包锦渊.  华西药学杂志. 2009(04)
[6]豆科锦鸡儿属（Caragana Fabr.）植物地理分布与分化研究[J]. 周道玮,刘钟龄,马毓泉.  植物研究. 2005(04)
[7]锦鸡儿属几种植物总黄酮含量研究[J]. 蒙秋霞,牛宇,牛西午.  华北农学报. 2005(03)
[8](6aR,11aR)-3-羟基-4,9-二甲氧基紫檀烷分子的晶体结构（英文）[J]. 邓雁如,江艳烽,席婵娟,武水仙,汪汉卿.  化学物理学报. 2004(04)



本文编号：2973704