白藜芦醇抗小鼠急性T淋巴细胞白血病作用及Notch1信号通路机制的研究
发布时间：2021-04-11 06:30
目的:观察白藜芦醇对急性T淋巴细胞白血病小鼠的作用及Notch1、Hes-1信号分子的表达水平,并探讨其作用机制。方法:1.完全随机法将50只C57BL/6雌性健康小鼠分为5组:正常对照组;模型对照组;白藜芦醇低浓度处理组（25mg/kg/d）;白藜芦醇中浓度处理组（50mg/kg/d）;白藜芦醇高浓度处理组（100mg/kg/d）。尾静脉注射1×106个GFP+白血病细胞,制备T-ALL白血病小鼠模型。2.应用瑞氏-吉姆萨染色观察各组外周血白血病细胞浸润情况。3.流式细胞术检测各实验组外周血、脾细胞悬液中白血病细胞的百分比。4.HE染色法观察各实验组肝脏、脾脏、肾脏、骨髓组织中白血病细胞的浸润情况。5.应用实时荧光定量PCR（RT-PCR）检测各组脾脏组织中Notch1、Hes-1信号分子mRNA的表达量。6.应用免疫蛋白印迹法（Western Blot）检测各组脾脏组织中Notch1、Hes-1蛋白表达水平。结果:1.模型对照组、白藜芦醇低、中、高浓度处理组的外周血涂片均可观察到原始淋巴细胞,其细胞核呈圆形或略呈卵圆形,大而深染,染色质致...
【文章来源】：河北医科大学河北省
【文章页数】：44 页
【学位级别】：硕士
【部分图文】：
57BL/6小鼠与T-ALL模型小鼠的一般情况
23影响Fig.2 Effect of resveratrol on leukemia cells of peripheral blood in micewith T-ALL by FCM.A: Original recordings of FCM. P2: The rates of GFP+cells.B: Comparison of GFP+cell rates among different groups at different times.a: control group; b: model group; c: 25mg/kg/d Res group; d: 50 mg/kg/d Res
图 3 流式细胞术检测白藜芦醇对 T-ALL 白血病小鼠脾细胞悬液中白血病细胞的影响Fig.3 Effect of resveratrol on leukemia cells of spleen cell suspension in micewith T-ALL by FCM.All data were expressed as mean ± SD; n=5 for each group.#P﹤0.05 vs.model group.AB
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期刊论文
[1]虎杖提取物白藜芦醇抗肿瘤机制的研究进展[J]. 何蕾,倪敏,樊志敏. 中医药导报. 2017(08)
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[3]Notch1信号通路与白藜芦醇抗急性T淋巴细胞白血病作用机制研究进展[J]. 李永军,史敏,秦永亮. 临床检验杂志. 2012(10)
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本文编号：3130761
【文章来源】：河北医科大学河北省
【文章页数】：44 页
【学位级别】：硕士
【部分图文】：
57BL/6小鼠与T-ALL模型小鼠的一般情况
23影响Fig.2 Effect of resveratrol on leukemia cells of peripheral blood in micewith T-ALL by FCM.A: Original recordings of FCM. P2: The rates of GFP+cells.B: Comparison of GFP+cell rates among different groups at different times.a: control group; b: model group; c: 25mg/kg/d Res group; d: 50 mg/kg/d Res
图 3 流式细胞术检测白藜芦醇对 T-ALL 白血病小鼠脾细胞悬液中白血病细胞的影响Fig.3 Effect of resveratrol on leukemia cells of spleen cell suspension in micewith T-ALL by FCM.All data were expressed as mean ± SD; n=5 for each group.#P﹤0.05 vs.model group.AB
【参考文献】：
期刊论文
[1]虎杖提取物白藜芦醇抗肿瘤机制的研究进展[J]. 何蕾,倪敏,樊志敏. 中医药导报. 2017(08)
[2]白藜芦醇逆转肿瘤耐药的分子机制研究进展[J]. 郭玉楷,史敏,秦永亮,李永军. 基础医学与临床. 2016(03)
[3]Notch1信号通路与白藜芦醇抗急性T淋巴细胞白血病作用机制研究进展[J]. 李永军,史敏,秦永亮. 临床检验杂志. 2012(10)
[4]Effect of resveratrol and in combination with 5-FU on murine liver cancer[J]. Sheng-Li Wu Zhong-Jie Sun Liang Yu Ke-Wei Meng Xing-Lei Qin Cheng-En Pan Department of Hepatobiliary Surgery,First Hospital of Xi’an Jiaotong University,Xi’an 710061,Shaanxi Province,ChinaDepartment of Hepatobiliary Surgery,People’s Hospital of Shaanxi Province,Xi’an 710061,Shaanxi Province,China. World Journal of Gastroenterology. 2004(20)
[5]Effect of resveratrol on cell cycle proteins in murine transplantable liver cancer[J]. Liang Yu Zhong-Jie Sun Sheng-Li Wu Cheng-En Pan, Department of Hepatobiliary Surgery, First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China. World Journal of Gastroenterology. 2003(10)
本文编号：3130761
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