淫羊藿苷经Wnt/β-catenin信号通路调控骨髓间充质干细胞的成骨—成脂双向分化

发布时间：2021-06-08 07:34

　　目的探讨Wnt/β-catennin信号通路介导的淫羊藿苷（Icariin）调控BMSCs（SD大鼠）成骨-成脂双向分化。方法1.体外实验（1）用细胞增殖、毒性试剂盒（CCK-8）检测BMSCs在多种不同浓度Icariin的干预其增殖活性的强弱及是细胞产生毒性反应。（2）用RT-q PCR确定Icariin对成骨分化指标（ALP、COL1、RUNX2）的影响,使用碱性磷酸酶（ALP）染色试剂盒和茜素红染色试剂确定经Icariin干预后ALP的活性及强弱、是否钙结节的形成及形成量（3）用RT-q PCR确定Icariin对成脂分化指标（PPARγ、Adipsin、FABP4）的影响,使用油红O染色试剂确定脂滴的形成。（4）使用信号通路特异性抑制蛋白DKK-1联合Icariin进行干预,检测Icariin在BMSCs成骨-成脂分化的作用。（5）Western-blot及Immunofluorescence检测Icariin干预后Wnt信号通路关键蛋白P-GSK、β-catennin表达和定位情况。2.在体试验用12周龄C57小鼠做卵巢切除模拟绝经后骨质疏松（OVX）,口服Icariin（2... 

【文章来源】：华中科技大学湖北省 211工程院校 985工程院校 教育部直属院校

【文章页数】：69 页

【学位级别】：硕士

【文章目录】：
前言
    参考文献
中文摘要
Abstract
引言
材料与方法
结果
讨论
参考文献
综述
    参考文献
附录一
附录二 研究生期间发表的论文
致谢


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期刊论文
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