川陈皮素通过调控RORα改善自然衰老小鼠骨流失（英文）
发布时间：2021-08-04 08:53
随着世界人口老龄化的加速,老年性骨质疏松症的发病率呈现出明显的上升趋势.衰老相关的骨质流失的机制研究对寻找有效的治疗骨质疏松药物具有重要意义.天然产物为治疗骨质疏松症药物的发现提供了丰富的来源.川陈皮素是柑橘属植物果实中含量最丰富的黄酮类成分之一,具有多种生物活性.在本研究中以20个月自然衰老小鼠为模型,连续15天腹腔注射给予川陈皮素,Micro-CT结果表明川陈皮素可以增加自然衰老小鼠骨体积分数和降低骨小梁分离度,改善自然衰老小鼠的骨微观结构,HE染色结果表明川陈皮素可增加骨小梁表面成骨细胞数量,川陈皮素处理小鼠血清中骨钙蛋白（Osteocalcin）水平显著增加.在细胞水平,川陈皮素处理小鼠前成骨细胞（MC3T3-E1）,Mki67水平显著增加,骨碱性磷酸酶水平和骨钙蛋白水平增加,茜素红染色明显增强,表明川陈皮素能促进MC3T3-E1细胞增殖、分化和矿化.进一步机制研究发现川陈皮素可直接激活视黄酸受体相关孤儿受体α（retinoic acid receptor-related orphan receptors,RORα）,并以RORα依赖的方式下调骨硬化蛋白（Sclerostin,...
【文章来源】：生物化学与生物物理进展. 2020,47(08)北大核心SCICSCD
【文章页数】：9 页
【文章目录】：
1 Materials and methods
1.1 Animals
1.2 Cell culture
1.3 HE staining
1.4 Alizarin red S staining
1.5 ALP(alkaline phosphatase)activity assay
1.6 Evaluation of serum markers of bone formation
1.7 Analysis of bone by micro-computed tomography(Micro-CT)
1.8 Western blot analysis
1.9 Quantitative real-time PCR assay
1.1 0 Data analysis
2 Results
2.1 Nobiletin improves bone microstructure and bone formation in natural aging mice
2.2 Nobiletin promotes osteoblast proliferation,differentiation and mineralization
2.3 Nobiletin regulates RORαand SOST in natural aging mouse
2.4 The effect of nobiletin on bone formation depends on RORα
3 Discussion
【参考文献】：
期刊论文
[1]川陈皮素改善衰老认知功能损伤的作用与机制（英文）[J]. 姚勤,张玉英,吴凯源,褚博煜,陈畅. 生物化学与生物物理进展. 2019(06)
[2]D-ribose increases triglyceride via upregulation of DGAT in the liver[J]. Yao Chen,Lexiang Yu,Yan Wei,Yang Long,Yong Xu,Tao He,Rongqiao He. Science China（Life Sciences）. 2019(06)
[3]Paracrine and endocrine actions of bone——the functions of secretory proteins from osteoblasts, osteocytes, and osteoclasts[J]. Yujiao Han,Xiuling You,Wenhui Xing,Zhong Zhang,Weiguo Zou. Bone Research. 2018(02)
本文编号：3321407
【文章来源】：生物化学与生物物理进展. 2020,47(08)北大核心SCICSCD
【文章页数】：9 页
【文章目录】：
1 Materials and methods
1.1 Animals
1.2 Cell culture
1.3 HE staining
1.4 Alizarin red S staining
1.5 ALP(alkaline phosphatase)activity assay
1.6 Evaluation of serum markers of bone formation
1.7 Analysis of bone by micro-computed tomography(Micro-CT)
1.8 Western blot analysis
1.9 Quantitative real-time PCR assay
1.1 0 Data analysis
2 Results
2.1 Nobiletin improves bone microstructure and bone formation in natural aging mice
2.2 Nobiletin promotes osteoblast proliferation,differentiation and mineralization
2.3 Nobiletin regulates RORαand SOST in natural aging mouse
2.4 The effect of nobiletin on bone formation depends on RORα
3 Discussion
【参考文献】：
期刊论文
[1]川陈皮素改善衰老认知功能损伤的作用与机制（英文）[J]. 姚勤,张玉英,吴凯源,褚博煜,陈畅. 生物化学与生物物理进展. 2019(06)
[2]D-ribose increases triglyceride via upregulation of DGAT in the liver[J]. Yao Chen,Lexiang Yu,Yan Wei,Yang Long,Yong Xu,Tao He,Rongqiao He. Science China（Life Sciences）. 2019(06)
[3]Paracrine and endocrine actions of bone——the functions of secretory proteins from osteoblasts, osteocytes, and osteoclasts[J]. Yujiao Han,Xiuling You,Wenhui Xing,Zhong Zhang,Weiguo Zou. Bone Research. 2018(02)
本文编号：3321407
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