花榈木提取物对慢性不可预知应激小鼠认知损害的影响（英文）
发布时间：2021-09-08 15:02
认知缺陷是抑郁症的核心症状之一,已成为抑郁症治疗面临的的一大挑战.花榈木为我国南方广泛分布的一种绿色乔木,具有抗抑郁作用.然而,花榈木对认知损害的作用尚未见报道.本研究旨在探讨花榈木提取物对慢性应激引起的认知障碍的影响,通过建立小鼠慢性不可预测轻度应激（CUMS）模型,评价花榈木改善认知功能的作用.认知行为采用新物识别和Morris水迷宫测试,免疫组织化学分析海马组织中GFAP和Aβ蛋白的水平,Western Blotting分析海马组织NLRP3、TNF-α和IL-1β蛋白表达.结果显示,花榈木提取物能明显增加小鼠的物体识别辨别指数,明显减少定位航行小鼠平台潜伏期,增加平台穿越次数.花榈木提取物能显著提高海马组织中5-HT、DA、NE的水平;同时提高应激小鼠海马CA1区GFAP水平,降低Aβ沉积,减少海马区NLRP3、TNF-α和IL-1β的表达.以上数据表明,花榈木提取物能明显改善CUMS小鼠学习记忆障碍,其作用机制与上调GAFP、减少Aβ沉积、缓解炎性有关.
【文章来源】：生物化学与生物物理进展. 2020,47(08)北大核心SCICSCD
【文章页数】：12 页
【文章目录】：
1 Materials and methods
1.1 Plant material and reagents
1.2 Animals
1.3 Establishment of chronic unpredictable mild stress model(CUMS)
1.4 Grouping and administration
1.5 Behavioral assessment
1.5.1 Novel object recognition test(NORT)
1.5.2 Morris water maze test(MWM)
1.6 Neurotransmitters in hippocampal tissue in CUMS mice
1.7 Histology
1.8 Immunohistochemistry
1.9 Western blot analysis
1.1 0 Statistical analysis
1.1 1 The experimental procedure
2 Results
2.1 OHP alleviated the deficits of recognition memory in CUMS mice
2.2 OHP ameliorated the deficits of spatial learning and memory in CUMS mice
2.3 Effects of OHP on neurotransmitters in hippocampal tissue in CUMS mice
2.4 The histopathology of hippocampus in CUMS mice
2.5 OHP decreased the expression of GFAP and Aβprotein in the hippocampus of CUMS mice
2.6 Effects of OHP on the expression of NLRP3,TNF-α，IL-1βin hippocampus of mice
3 Discussion
4 Conclusion
【参考文献】：
期刊论文
[1]Nature brings new avenues to the therapy of central nervous system diseases——an overview of possible treatments derived from natural products[J]. Junchi Zhang,Yang He,Xiangrui Jiang,Hualiang Jiang,Jingshan Shen. Science China（Life Sciences）. 2019(10)
[2]Celastrol specifically inhibits the activation of NLRP3 inflammasome[J]. Xuesong Sang,Yihui Chen,Wei Chen,Jing Xie,Guangxun Meng,Jin Zhong,Taisheng Li,Ailing Lu. Science China（Life Sciences）. 2018(03)
[3]花梨木精油通过嗅觉通路改善小鼠抑郁样行为及其神经递质[J]. 陈军,徐金勇,徐蓉,刘莉茵,任振华,李光武. 江苏医药. 2012(06)
本文编号：3390997
【文章来源】：生物化学与生物物理进展. 2020,47(08)北大核心SCICSCD
【文章页数】：12 页
【文章目录】：
1 Materials and methods
1.1 Plant material and reagents
1.2 Animals
1.3 Establishment of chronic unpredictable mild stress model(CUMS)
1.4 Grouping and administration
1.5 Behavioral assessment
1.5.1 Novel object recognition test(NORT)
1.5.2 Morris water maze test(MWM)
1.6 Neurotransmitters in hippocampal tissue in CUMS mice
1.7 Histology
1.8 Immunohistochemistry
1.9 Western blot analysis
1.1 0 Statistical analysis
1.1 1 The experimental procedure
2 Results
2.1 OHP alleviated the deficits of recognition memory in CUMS mice
2.2 OHP ameliorated the deficits of spatial learning and memory in CUMS mice
2.3 Effects of OHP on neurotransmitters in hippocampal tissue in CUMS mice
2.4 The histopathology of hippocampus in CUMS mice
2.5 OHP decreased the expression of GFAP and Aβprotein in the hippocampus of CUMS mice
2.6 Effects of OHP on the expression of NLRP3,TNF-α，IL-1βin hippocampus of mice
3 Discussion
4 Conclusion
【参考文献】：
期刊论文
[1]Nature brings new avenues to the therapy of central nervous system diseases——an overview of possible treatments derived from natural products[J]. Junchi Zhang,Yang He,Xiangrui Jiang,Hualiang Jiang,Jingshan Shen. Science China（Life Sciences）. 2019(10)
[2]Celastrol specifically inhibits the activation of NLRP3 inflammasome[J]. Xuesong Sang,Yihui Chen,Wei Chen,Jing Xie,Guangxun Meng,Jin Zhong,Taisheng Li,Ailing Lu. Science China（Life Sciences）. 2018(03)
[3]花梨木精油通过嗅觉通路改善小鼠抑郁样行为及其神经递质[J]. 陈军,徐金勇,徐蓉,刘莉茵,任振华,李光武. 江苏医药. 2012(06)
本文编号：3390997
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