雷公藤甲素对大鼠坐骨神经冷保存后细胞活性及异体移植后神经再生的影响
发布时间：2022-02-04 18:37
目的探讨雷公藤甲素（T10）对冷保存大鼠坐骨神经生物活性和异体移植后神经再生的影响。方法取对数生长期施万细胞（SCs）,CCK-8检测10-66 mol/L、10-77 mol/L、10-88 mol/L、10-99 mol/L T10溶液对SCs增殖的影响。取Sprague-Dawley雌性大鼠双侧坐骨神经15 mm,分别在含0 mol/L、10-66 mol/L、10-7mol/L、10-88 mol/L、10-99 mol/L T10的DMEM液中,4℃或37℃培养24 h（n=6）,Western blotting检测神经生长因子（NGF）、神经胶质细胞源性神经营养因子（GDNF）、脑源性神经营养因子（BDNF）表达。另取大鼠坐骨神经15 mm,随机分成新鲜神经组（A组,n=30）、DMEM保存组（B组,n=30）、T10保存组（C组,n=30）、T10预处理后DMEM保存组（D组,n=30）、T10预处...
【文章来源】：重庆医科大学重庆市
【文章页数】：52 页
【学位级别】：硕士
【部分图文】：
雷公藤甲素（T10）对雪旺细胞的细胞毒性Fig1.CytotoxicityofT10onSCs.Comparisonbetweenthe10-6MT10-treatedand
图 2: 雷公藤甲素预处理诱导培养坐骨神经 NGF、BDNF、GDNF 蛋白表达Fig 2. Levels of NGF, BDNF, and GDNF in cultured sciatic nerves pretreated withT10. Note :1) Significantly increased levels of NGF, BDNF, and GDNF were detectedfor all of the 37°C pretreatment groups compared with those for all of the 4°Cpretreatment groups (P<0.001, P<0.001, and P<0.001, respectively, Fig. 2A-C). 2) Forthe 4°C pretreatment groups, the 10-8M T10-treated group had significantly increasedNGF levels and increased levels of BDNFcompared with the groups receiving otherconcentrations of T10 (*P<0.001and *P=0.03, Fig. 2A and 2C).But no obviousdifferences were observed for GDNF expression levels among the groups (*P=0.147,Fig. 2B). 3) For the 37°C pretreatment groups, the 10-8M T10-treated groups hadsignificantly increased levels of NGF、GDNF and BDNF expression compared withthe other T10-treated groups ( **P<0.001, P<0.001, and P<0.001, respectively, Fig.2A-C).
D 组 E 组图 3：激光共聚焦显微镜观察冷保存神经细胞活性Fig 3. Observation of cryopreserved nerve cell activity using laser confocalmicroscopy.
【参考文献】：
期刊论文
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[2]Analysis of transcriptome sequencing of sciatic nerves in Sprague-Dawley rats of different ages[J]. Jiang-Hui Liu,Qing Tang,Xiang-Xia Liu,Jian Qi,Rui-Xi Zeng,Zhao-Wei Zhu,Bo He,Yang-Bin Xu. Neural Regeneration Research. 2018(12)
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本文编号：3613732
【文章来源】：重庆医科大学重庆市
【文章页数】：52 页
【学位级别】：硕士
【部分图文】：
雷公藤甲素（T10）对雪旺细胞的细胞毒性Fig1.CytotoxicityofT10onSCs.Comparisonbetweenthe10-6MT10-treatedand
图 2: 雷公藤甲素预处理诱导培养坐骨神经 NGF、BDNF、GDNF 蛋白表达Fig 2. Levels of NGF, BDNF, and GDNF in cultured sciatic nerves pretreated withT10. Note :1) Significantly increased levels of NGF, BDNF, and GDNF were detectedfor all of the 37°C pretreatment groups compared with those for all of the 4°Cpretreatment groups (P<0.001, P<0.001, and P<0.001, respectively, Fig. 2A-C). 2) Forthe 4°C pretreatment groups, the 10-8M T10-treated group had significantly increasedNGF levels and increased levels of BDNFcompared with the groups receiving otherconcentrations of T10 (*P<0.001and *P=0.03, Fig. 2A and 2C).But no obviousdifferences were observed for GDNF expression levels among the groups (*P=0.147,Fig. 2B). 3) For the 37°C pretreatment groups, the 10-8M T10-treated groups hadsignificantly increased levels of NGF、GDNF and BDNF expression compared withthe other T10-treated groups ( **P<0.001, P<0.001, and P<0.001, respectively, Fig.2A-C).
D 组 E 组图 3：激光共聚焦显微镜观察冷保存神经细胞活性Fig 3. Observation of cryopreserved nerve cell activity using laser confocalmicroscopy.
【参考文献】：
期刊论文
[1]Tissue engineering for the repair of peripheral nerve injury[J]. Pei-Xun Zhang,Na Han,Yu-Hui Kou,Qing-Tang Zhu,Xiao-Lin Liu,Da-Ping Quan,Jian-Guo Chen,Bao-Guo Jiang. Neural Regeneration Research. 2019(01)
[2]Analysis of transcriptome sequencing of sciatic nerves in Sprague-Dawley rats of different ages[J]. Jiang-Hui Liu,Qing Tang,Xiang-Xia Liu,Jian Qi,Rui-Xi Zeng,Zhao-Wei Zhu,Bo He,Yang-Bin Xu. Neural Regeneration Research. 2018(12)
[3]Decellularized sciatic nerve matrix as a biodegradable conduit for peripheral nerve regeneration[J]. Jongbae Choi,Jun Ho Kim,Ji Wook Jang,Hyun Jung Kim,Sung Hoon Choi,Sung Won Kwon. Neural Regeneration Research. 2018(10)
[4]冷诱导RNA结合蛋白促进冷冻保存大鼠坐骨神经异体移植后神经再生的作用[J]. 李子健,黄英如,曾欢欢,汪一,张松. 中国康复理论与实践. 2018(04)
[5]Stimulating effect of thyroid hormones in peripheral nerve regeneration:research history and future direction toward clinical therapy[J]. I.Barakat-Walter,R.Kraftsik. Neural Regeneration Research. 2018(04)
[6]Autologous transplantation with fewer fibers repairs large peripheral nerve defects[J]. Jiu-xu Deng,Dian-yin Zhang,Ming Li,Jian Weng,Yu-hui Kou,Pei-xun Zhang,Na Han,Bo Chen,Xiao-feng Yin,Bao-guo Jiang. Neural Regeneration Research. 2017(12)
[7]中医药促进周围神经损伤修复的研究进展[J]. 陈传奇,陈龙菊. 时珍国医国药. 2016(09)
[8]周围神经损伤后轴突再生微环境的研究进展[J]. 范红石,王艳,陈国平. 中国康复理论与实践. 2015(03)
[9]What’s new in clinical solid organ transplantation by 2013[J]. Maurizio Salvadori,Elisabetta Bertoni. World Journal of Transplantation. 2014(04)
[10]中医治疗周围神经损伤探讨[J]. 李玲. 中医研究. 2014(08)
本文编号：3613732
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