中西医结合治疗免疫性狼疮鼠的实验研究

发布时间：2018-02-08 22:22

本文关键词： 狼疮性肾炎清热解毒补肾方细菌脂多糖(LPS) 抗核抗体肾脏免疫复合物　出处：《山东中医药大学》2007年博士论文　论文类型：学位论文

【摘要】： 第一部分SLE小鼠模型的建立目的:探讨腹腔注射细菌脂多糖(LPS)制造SLE小鼠模型的最佳剂量。方法:80只小鼠随机分为4组,分别腹腔注射LPS 5 mg·kg - 1、2.5 mg·kg - 1和1.25 mg·kg - 1和生理盐水。结果:腹腔注射LPS 5 mg·kg - 1、2.5 mg·kg - 1组动物肾脏病理改变符合SLE病理特点,动物24小时尿蛋白含量、血清ANA的滴度、肾脏免疫复合物光密度均明显升高,而腹腔注射LPS 5 mg·kg - 1组动物死亡率明显升高。结果:腹腔注射LPS 5 mg·kg- 1、2.5 mg·kg - 1均能制造出良好的狼疮性肾炎模型,而腹腔注射LPS 5 mg·kg- 1动物死亡率高。第二步分中西医结合治疗对SLE模型鼠疗效观察目的:探讨中西医结合治疗对SLE模型鼠的疗效。方法:120只小鼠腹腔注射2.5 mg·kg - 1制造SLE模型鼠,随机分为6组,分别给予蒸馏水、中药清热解毒补肾方、强的松、环磷酰胺、清热解毒补肾方+强的松、清热解毒补肾方+环磷酰胺在造模的同时灌胃治疗15天。结果:与对照组相比,各治疗组动物死亡率、24小时尿蛋白含量、血清ANA的滴度、肾小球免疫复合物沉积均有不同程度的下降,以中西医结合治疗组降低最为明显。结论:中药清热解毒补肾方及西药强的松、环磷酰胺对狼疮样小鼠模型均有良好的治疗作用,而中西医结合治疗效果最好。
[Abstract]:Part I Establishment of SLE Mouse Model. Objective: to study the optimal dosage of intraperitoneal injection of lipopolysaccharide (LPS) to make SLE mouse model. Methods: 80 mice were randomly divided into 4 groups. LPS 5 mg 路kg -1 and 1.25 mg 路kg -1 and normal saline were injected intraperitoneally respectively. Results: the pathological changes of kidney in LPS 5 mg 路kg -1 + 2.5 mg 路kg -1 group were in accordance with the pathological characteristics of SLE. The urine protein content and the titer of serum ANA were observed in 24 hours after intraperitoneal injection of LPS 5 mg 路kg -1 and 2.5 mg 路kg -1 LPS, respectively. The light density of renal immune complex was significantly increased, but the mortality of LPS 5 mg 路kg -1 group was significantly increased. Results: intraperitoneal injection of LPS 5 mg 路kg -1 and 2.5 mg 路kg -1 could produce a good model of lupus nephritis. However, intraperitoneal injection of LPS 5 mg 路kg -1 resulted in high mortality. The second step is to observe the therapeutic effect of integrated traditional Chinese and western medicine on SLE model rats. Objective: to investigate the therapeutic effect of integrated traditional Chinese and western medicine on SLE model mice. Methods: SLE model mice were induced by intraperitoneal injection of 2.5 mg 路kg -1 in 1: 120 mice. The mice were randomly divided into six groups: distilled water, traditional Chinese medicine to clear heat and detoxify kidney, prednisone, cyclophosphamide. Results: compared with the control group, the 24 hour urine protein content and serum ANA titer of each treatment group were compared with those of the control group, which were treated by oral administration of cyclophosphamide at the same time. The deposition of glomerular immune complex decreased in varying degrees, especially in the treatment group of integrated traditional Chinese and western medicine. Conclusion: the traditional Chinese medicine for clearing heat and detoxifying kidney and the western medicine prednisone and cyclophosphamide have good therapeutic effects on the model of lupus like mice. And the combination of traditional Chinese and western medicine treatment effect is the best.
【学位授予单位】：山东中医药大学
【学位级别】：博士
【学位授予年份】：2007
【分类号】：R593.241

【参考文献】