Numb和GPC3在原发性肝细胞肝癌中的表达及临床意义

发布时间：2018-01-29 02:46

  本文关键词： 原发性肝细胞肝癌 Numb GPC3 相关性 预后　出处：《郑州大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的探讨Numb和GPC3在原发性肝细胞肝癌中的表达及二者的相关性,探究Numb和GPC3在原发性肝细胞肝癌中的临床价值。方法选择郑州大学第一附属医院肝胆胰外科于2008年6月至2011年6月收治的原发性肝细胞肝癌患者共60例,同时选取27例相同时间段的肝血管瘤及肝外伤的患者。分别统计分析,Numb与GPC3在原发性肝细胞肝癌组织、癌旁组织、正常组织中的表达情况,同时对比Numb和GPC3阳性表达率与各组患者临床资料的关系,以及Numb和GPC3在三种组织中的表达相关性。结果原发性肝细胞肝癌组织中Numb蛋白主要呈现阴性,癌旁组织中Numb蛋白主要呈现阴性或弱阳性,正常肝组织中主要呈现弱阳性或阳性,三组间阳性表达率差异有统计学意义(PO.05)。对比临床资料,Numb蛋白表达水平与三组患者的肝硬化程度、肿瘤大小,年龄及性别无关,差异无统计学意义(P0.05),而与患者Edmondson分级及肿瘤包膜、TNM分期及门脉癌栓有关,差异均有统计学意义(P0.05)。即Edmondson分级III~Ⅳ级、TNM分期Ⅲ期、无肿瘤包膜但有门脉癌栓的患者,Numb蛋白在三组患者中主要呈现阴性或弱阳性,三组患者之间阳性表达率差异具有统计学意义(P0.05)。同时,相对于正常肝脏组织来说原发性肝细胞肝癌组织中GPC3的阳性表达率则大大升高,差异具有统计学意义(P0.05)。患者的肝硬化程度、肿瘤大小、年龄、性别则与GPC3的表达水平无关,差异无统计学意义(P0.05)。而与患者Edmondson分级及肿瘤包膜、TNM分期及门脉癌栓有关,差异均有统计学意义(P0.05)。即对于Edmondson分级处于Ⅲ~Ⅳ级及无肿瘤包膜、TNM分期处于III期及存在门脉癌栓的患者,GPC3阳性率将明显提高,差异有统计学意义(P0.05)。GPC3蛋白表达与Numb表达呈负相关(P0.05)。结论原发性肝细胞肝癌的侵袭性与Numb蛋白阳性表达率下降及GPC3阳性表达率上升均相关,且二者负相关表达。因而对原发性肝细胞肝癌患者来说,Numb蛋白与GPC3的表达对其预后的判定可能具有一定的临床指导作用。
[Abstract]:Objective to investigate the expression of Numb and GPC3 in hepatocellular carcinoma (HCC) and their correlation. To explore the clinical value of Numb and GPC3 in primary hepatocellular carcinoma (HCC). Methods Primary hepatic fine liver was selected from June 2008 to June 2011 in the Department of Hepatobiliary and Pancreatic surgery, the first affiliated Hospital of Zhengzhou University. There were 60 patients with hepatocellular carcinoma. At the same time, 27 patients with hepatic hemangioma and liver trauma were selected to analyze the expression of GPC3 and numb in primary hepatocellular carcinoma (HCC), paracancerous tissues and normal tissues respectively. At the same time, the relationship between the positive expression rate of Numb and GPC3 and the clinical data of each group was compared. The expression of Numb and GPC3 in three tissues were correlated. Results Numb protein was negative in primary hepatocellular carcinoma (HCC). Numb protein was mainly negative or weakly positive in paracancerous tissues and weakly positive or positive in normal liver tissues. The difference of positive expression rate among the three groups was statistically significant (P < 0.05). The clinical data were compared. The expression of Numb protein was not related to the degree of cirrhosis, tumor size, age and sex, but there was no significant difference between the three groups (P 0.05). However, there were significant differences in Edmondson grade, tumor capsule TNM stage and portal vein tumor thrombus. The difference was significant (P 0.05), that is, Edmondson grade III- 鈪，

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