黑色素瘤缺乏因子2在肝癌以及癌旁组织中的表达分析

发布时间：2018-02-02 02:44

  本文关键词： 肝癌 癌旁 黑色素瘤缺乏因子2 半胱天冬酶-1 白介素-1β　出处：《山东大学》2015年硕士论文　论文类型：学位论文

【摘要】：[背景／目的]慢性乙型肝炎相关性肝癌的发病机制目前尚不明确,目前认为其发病主要与乙肝病毒与宿主基因整合、慢性肝脏炎症有关。近年来,发现有很多细胞因子参与了慢性乙型肝炎的炎症反应过程,其中AIM2是目前研究的热点之一。AIM2(黑色素瘤缺乏因子2)是一种主要定位于细胞质的蛋白质,它可以与细胞质双链DNA结合,诱导ASC(凋亡相关的斑点样蛋白)炎性复合体的形成,激活caspase-1(半胱天冬酶-1),促进IL-1β(白细胞介素-1β)的释放,诱导机体固有免疫反应,抵御病毒、细菌的感染。本研究旨在探讨AIM2在慢性乙型肝炎相关性肝癌的发生和发展过程中的作用。[方法]50例肝癌患者手术切除的肝癌以及癌旁组织,肝癌的诊断符合病理学诊断标准。所有患者HBsAg阳性且均未见远隔转移灶,在手术前均未进行过化疗或放疗,亦未进行过免疫抑制剂治疗。排除合并其他病毒感染所致的肝炎。用免疫组织化学方法分别测定肝癌和癌旁组织中AIM2、caspase-1、IL-1β的表达。组间比较应用卡方检验和秩和检验,相关性分析应用spearman相关分析。[结果]AIM2在肝癌组织中的阳性表达率高于癌旁组织(X2=8.274,P0.05),肝癌组织中AIM2的表达强度明显高于癌旁组织(Z。=3.243,P0.05)。e抗原表达阳性者AIM2表达强度均高于阴性者(肝癌：Z。=2.620,P0.05；癌旁：Z。=2.367,P0.05)。高病毒载量患者(HBV-DNAS≥1×105copies/ml)中AIM2的表达显著高于低病毒载量患者(HBV-DNA1×105copies/ml)(肝癌组织：Zc=3.242,P0.05；癌旁组织：Z。=3.379,P0.05)。肝癌组织中AIM2的表达与ALT、AST、AFP水平的高低有关(rs=0.333,0.548,0.336, P0.05)。Caspase-1的表达与AIM2的表达呈正相关(肝癌：rs=O.903,p0.01；癌旁：rs=O.910,p0.01)；IL-1 β的表达与AIM2的表达呈正相关(肝癌rs=0.905,p0.01；癌旁：rs=0.841,p0.01)。[结论]1.AIM2在肝癌组织中的阳性表达主要位于细胞质中,肝癌组织中AIM2的表达程度高于癌旁组织。2.HBV病毒载量高的患者AIM2表达程度较高, e抗原阳性的患者AIM2表达程度较高, AIM2表达程度与AST、ALT水平呈正相关性,AIM2的表达与患者的年龄、性别、PLT以及白蛋白水平无关。3.AIM2的表达程度与AFP的水平呈正相关性,而与肝癌分级无关。
[Abstract]:[Background / objective: the pathogenesis of chronic hepatitis B associated liver cancer is still unclear. It is believed that the pathogenesis is mainly related to the integration of hepatitis B virus with host genes and chronic liver inflammation. Many cytokines were found to be involved in the inflammatory response of chronic hepatitis B. AIM2 (melanoma deficiency factor 2) is a major cytoplasmic protein, which can bind to cytoplasmic double-stranded DNA. Asc (apoptosis-related dot-like protein) inflammatory complex was induced, caspase-1 (caspase-1) was activated and IL-1 尾 (interleukin-1 尾) was released. The purpose of this study was to investigate the role of AIM2 in the occurrence and development of chronic hepatitis B related liver cancer. [Methods] the diagnosis of liver cancer in 50 patients with liver cancer was in accordance with the pathological diagnostic criteria. HBsAg positive and no distant metastasis was found in all the patients. No chemotherapy or radiotherapy or immunosuppressive therapy was performed before the operation. Hepatitis caused by other virus infection was excluded. AIM2 in liver cancer and adjacent tissues were determined by immunohistochemical method. Expression of caspase-1 and IL-1 尾. Chi-square test and rank sum test were used in the comparison between groups. Correlation analysis was performed with spearman correlation analysis. [Results the positive expression rate of AIM2 in HCC tissues was higher than that in paracancerous tissues (P 0.05). The expression of AIM2 in HCC tissues was significantly higher than that in adjacent tissues. The expression intensity of AIM2 was higher in the positive expression of P0.05n.e antigen than that in the negative group (P0.05). Next to cancer: Z. 2.367. The expression of AIM2 in patients with high viral load was significantly higher than that in patients with low viral load (HBV-DNA S 鈮，

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