IL-4激活的巨噬细胞通过分泌CCL18促进Hela细胞侵袭和迁移

发布时间：2018-02-07 09:54

本文关键词： 宫颈癌 IL-激活的巨噬细胞 CCL NF-κB 侵袭转移　出处：《中山大学学报(医学科学版)》2017年01期 　论文类型：期刊论文

【摘要】：【目的】研究IL-4激活的巨噬细胞对宫颈癌Hela细胞的侵袭、转移作用及其机制。【方法】细胞形态观察、流式细胞术及ELISA检测巨噬细胞的激活状态。Transwell法观察Hela细胞的侵袭转移能力。Western blot检测NF-κB信号蛋白的激活状况及转移相关基因的表达。【结果】(1)IL-4激活的巨噬细胞表现为免疫抑制的M2型表型;(2)IL-4激活的巨噬细胞显著促进Hela细胞的侵袭、迁移,而未激活的巨噬细胞没有这种能力(P0.001);(3)IL-4激活的巨噬细胞上清中加入CCL18中和抗体而不是同型Ig G对照能削弱M2型巨噬细胞的作用(P0.001);(4)IL-4激活的巨噬细胞上清能促进共培养肿瘤细胞中NF-κB信号蛋白的激活和vimentin的表达;(5)直接应用CCL18也可以促进Hela细胞的侵袭转移,NF-κB信号蛋白的激活和vimentin的表达。【结论】IL-4激活的巨噬细胞可以促进宫颈癌细胞的侵袭转移,其作用机制为释放CCL18作用于肿瘤细胞的NF-κB通路,进而促进转移相关基因的表达。
[Abstract]:[objective] to study the invasion and metastasis of IL-4 activated macrophages on cervical cancer Hela cells and its mechanism. Flow cytometry and ELISA were used to detect the activation of macrophages. Transwell method was used to observe the invasion and metastasis ability of Hela cells. Western blot was used to detect the activation of NF- 魏 B signal protein and the expression of metastasis-associated genes. [results] the expression of IL-4 activated macrophages was as follows. Immunosuppressive M2 phenotype and IL-4 activated macrophages significantly promoted the invasion of Hela cells. Migration, However, the unactivated macrophages had no such ability. The addition of CCL18 neutralizing antibody to the supernatant of macrophages activated by P0.001 and IL-4, rather than the control of the same type IgG, could weaken the effect of M2 macrophages. P0.001P001H4 IL-4 activated macrophage supernatant could promote co-culture. The activation of NF- 魏 B signal protein and the expression of vimentin in tumor cells. (5) the direct application of CCL18 can also promote the activation of NF- 魏 B signal protein and the expression of vimentin in Hela cells. [conclusion] the macrophages activated by IL-4 can promote the invasion and metastasis of cervical cancer cells. The mechanism is the release of CCL18 into NF- 魏 B pathway of tumor cells, which promotes the expression of metastasis-associated genes.
【作者单位】：上海市第八人民医院妇产科;中山大学孙逸仙纪念医院乳腺肿瘤医学部;
【基金】：高校基本科研业务费中山大学青年教师培育项目(14ykpy20) 高等学校博士学科点专项科研基金(新教师类)(20110171120082)
【分类号】：R737.33

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