网络筛选乳腺癌相关miRNAs及miR-106a-5p对乳腺癌细胞侵袭迁移能力的影响

发布时间：2018-02-09 17:59

本文关键词： miR-a-p 生物信息学乳腺癌细胞侵袭迁移　出处：《第三军医大学学报》2017年02期 　论文类型：期刊论文

【摘要】：目的网络筛选乳腺癌关键微小RNAs(micro RNAs,miRNAs),并探讨miR-106a-5p对乳腺癌细胞侵袭及迁移能力的影响。方法通过Target Scan和miRanda等工具,构建miRNAs与靶基因以及显著靶向性功能(gene ontology,GO)的调控网络,筛选对乳腺癌有关键调控作用的miRNAs;利用miR-106a-5p agomir及miR-106a-5p antagomir转染MCF-7、T47D乳腺癌细胞株,建立乳腺癌细胞中miR-106a-5p的过表达及敲低体系,Transwell侵袭实验、Transwell迁移实验、划痕实验检测miR-106a-5p对乳腺癌细胞的侵袭、迁移能力的影响。结果在miRNAs-gene-network和miRNAs-GO-network两个网络中调控维度最高的miRNAs基本一致,其中miR-106a-5p是调控维度最高的miRNAs之一;过表达miR-106a-5p显著抑制乳腺癌细胞的侵袭能力和迁移能力(P0.05),敲低miR-106a-5p则显著增加乳腺癌细胞的侵袭能力和迁移能力(P0.05)。结论 miR-106a-5p是乳腺癌miRNAs调控网络中的关键miRNAs之一,具有抑制乳腺癌细胞的侵袭及迁移能力的生物学功能。
[Abstract]:Objective to screen the key tiny RNAs(micro RNAsmiRNAsG in breast cancer, and to explore the effect of miR-106a-5p on the invasion and migration of breast cancer cells. Methods the regulatory network of miRNAs and target genes and significantly targeted functional gene ontologygo was constructed by means of Target Scan and miRanda. MiR-106a-5p agomir and miR-106a-5p antagomir were used to transfect MCF-7G T47D breast cancer cell line, and to establish transwell migration assay for overexpression of miR-106a-5p and transwell invasion assay in breast cancer cells. The effect of miR-106a-5p on the invasion and migration of breast cancer cells was detected by scratch test. Results the miRNAs with the highest regulatory dimension was basically the same in miRNAs-gene-network and miRNAs-GO-network networks, among which miR-106a-5p was one of the miRNAs with the highest regulatory dimension. Overexpression of miR-106a-5p significantly inhibited the invasion and migration ability of breast cancer cells (P0.05A), while knockdown of miR-106a-5p significantly increased the invasion and migration ability of breast cancer cells (P0.05G). Conclusion miR-106a-5p is one of the key miRNAs in the miRNAs regulatory network of breast cancer. It has the biological function of inhibiting the invasion and migration of breast cancer cells.
【作者单位】：第三军医大学新桥医院肿瘤科;
【基金】：国家自然科学基金面上项目(81272910)~~
【分类号】：R737.9

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