MicroRNA-25表达下调对神经胶质瘤U87细胞增殖与凋亡的影响

发布时间：2018-02-15 15:07

本文关键词： 神经胶质瘤 microRNA- B细胞淋巴瘤/白血病-基因增殖凋亡　出处：《中国现代医学杂志》2017年22期 　论文类型：期刊论文

【摘要】：目的探讨micro RNA-25(mi R-25)表达下调对神经胶质瘤U87细胞增殖与凋亡的影响,以及mi R-25与B细胞淋巴瘤/白血病-2基因(Bcl-2)在胶质瘤细胞增殖与凋亡过程中的调节作用。方法通过慢病毒介导反义mi R-25寡聚核苷酸转染U87细胞;分为3组,实验组(转染anti-mi R-25)、对照组(转染negative control)和空白组(空白PBS);实时荧光定量聚合酶链反应(q RT-PCR)检测mi R-25和Bcl-2的m RNA表达水平;CCK-8法检测细胞增殖情况;流式细胞术检测细胞周期和细胞凋亡。结果实验组mi R-25和Bcl-2的m RNA表达量与空白组和对照组比较,差异具有统计学意义(P0.05),实验组降低;实验组相对于空白组和对照组,细胞增殖活性降低,细胞周期延缓,细胞凋亡率升高。结论在胶质瘤U87细胞,mi R-25表达下调通过作用于Bcl-2靶基因,延缓细胞周期,抑制细胞增殖,促进细胞凋亡。
[Abstract]:Objective to investigate the effect of down-regulation of micro RNA-25(mi R-25 on proliferation and apoptosis of glioma U87 cells. And the role of mi R-25 and B cell lymphoma / leukemia 2 gene Bcl-2 in the proliferation and apoptosis of glioma cells. Methods U87 cells were transfected with antisense miR-25 oligonucleotides mediated by lentivirus and divided into three groups. In the experimental group (transfected with anti-mi R-25), the control group (transfected with negative control) and the blank group (control group), the expression level of m RNA of miR-25 and Bcl-2 was detected by real-time quantitative polymerase chain reaction (RT-PCR) and the cell proliferation was detected by CCK-8 method. Results the expression of m RNA of miR-25 and Bcl-2 in the experimental group was significantly lower than that in the blank group and the control group (P 0.05), while the expression of m RNA in the experimental group was lower than that in the blank group and the control group. Conclusion in U87 glioma cells, the down-regulated expression of miR-25 inhibits cell cycle, inhibits cell proliferation and promotes apoptosis by acting on Bcl-2 target gene.
【作者单位】：华北理工大学附属医院神经外科;河北医科大学附属邢台市人民医院神经外科;
【分类号】：R739.41

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