γ-生育三烯酚抑制人胃腺癌SGC-7901细胞侵袭和迁移及其机制探讨

发布时间：2018-02-19 22:12

本文关键词： 胃肿瘤生育三烯酚类肿瘤浸润细胞迁移分析 NF-κB　出处：《肿瘤》2017年05期 　论文类型：期刊论文

【摘要】：目的:探讨γ-生育三烯酚(γ-tocotrienol,γ-T3)对人胃腺癌SGC-7901细胞侵袭和迁移的抑制作用及其可能的分子机制。方法:用不同浓度(0、15、30、45、60和100μmol/L)的γ-T3作用SGC-7901细胞后,采用CCK-8法、细胞划痕愈合实验和Transwell侵袭实验分别检测细胞增殖、迁移和侵袭能力的变化,然后采用蛋白质印迹法检测细胞中环氧合酶2(cyclooxygenase-2,COX-2)和核因子κB(nuclear factor-kappa B,NF-κB)信号通路蛋白的表达。结果:15~100μmol/Lγ-T3作用SGC-7901细胞24、48和72 h后,细胞增殖能力受到明显抑制,且呈时间和剂量依赖性(P值均0.01)。15~60μmol/Lγ-T3单独作用或与10 ng/mL肿瘤坏死因子α(tumor necrosis factor-alpha,TNF-α)联合作用SGC-7901细胞24 h后,细胞迁移和侵袭能力均被明显抑制(P值均0.01)。15~60μmol/Lγ-T3作用SGC-7901细胞24 h后,NF-κB、NF-κB p65和COX-2蛋白的表达水平均随着作用浓度的增加呈明显降低趋势(P值均0.01)。结论:γ-T3可抑制人胃腺癌SGC-7901细胞的侵袭和转移,其作用机制可能与阻滞NF-κB信号通路和下调COX-2蛋白表达有关。
[Abstract]:Objective: to investigate the inhibitory effect of 纬 -tocotrienol (纬 -T3) on the invasion and migration of human gastric adenocarcinoma SGC-7901 cells and its possible molecular mechanism. Methods: SGC-7901 cells were treated with 纬 -T 3 at different concentrations and 100 渭 mol / L respectively. Cell scratch healing test and Transwell invasion assay were used to detect the changes of cell proliferation, migration and invasion ability, respectively. Then the expression of cyclooxygenase-2COX-2) and NF- 魏 B nuclear factor-kappa factor-kappa NF- 魏 B signaling pathway proteins were detected by Western blot. Results the proliferation of SGC-7901 cells was significantly inhibited after treated with 100 渭 mol/L 纬 -T3 for 2448 h and 72 h. In a time-and dose-dependent manner, both 0. 01 渭 mol/L 纬 -T3 and 10 ng/mL TNF- 伪 necrosis factor-alpha TNF- 伪 treated SGC-7901 cells alone or in combination with 10 ng/mL tumor necrosis factor-alpha (TNF- 伪) for 24 h. The migration and invasion ability of SGC-7901 cells were significantly inhibited by P value of 0.01 渭 mol/L 纬 -T3 and the expression of NF- 魏 B p65 and COX-2 protein were decreased with the increase of the concentration. Conclusion: 纬 -T3 can inhibit the expression of SGC-7901 in human gastric adenocarcinoma. Cell invasion and metastasis, The mechanism may be related to blocking NF- 魏 B signaling pathway and down-regulating the expression of COX-2 protein.
【作者单位】：上海交通大学附属第六人民医院临床营养科;上海交通大学附属第六人民医院东院中心实验室;
【基金】：国家自然科学基金面上项目(编号:81273061)~~
【分类号】：R735.2

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