前列腺癌基因表达谱芯片的生物信息学分析

发布时间：2018-02-28 23:27

本文关键词： 前列腺癌基因表达谱生物信息学　出处：《安徽医科大学学报》2017年02期 　论文类型：期刊论文

【摘要】：目的利用生物信息学方法分析前列腺癌基因表达谱芯片,研究前列腺癌差异表达基因的功能及调控网络。方法采用GEO数据库中获取的前列腺癌基因表达谱芯片数据,利用R软件及affy、limma、pheatmap、ggplot2等R软件包进行数据挖掘及生物信息学分析。结合生物信息学工具DAVID、Gene MANIA对差异表达基因及其调控网络进行分析。结果共筛选出前列腺癌与癌旁组织差异表达基因56个,表达上调15个,表达下调41个,前列腺癌与癌旁组织的差异表达基因被富集到不同的子集。其中cav1、slc16a2、cav2、slc16a5、magi2、ptrf、pdlim5、lmod1、abcc6等9个基因被富集到"细胞功能"分类下的"细胞膜组分"子集中,其中cav1、cav2、ptrf影响细胞膜内陷囊状结构的功能,可能在前列腺癌发生发展中发挥重要作用。结论前列腺癌与癌旁组织的差异表达基因之间存在复杂的调控网络,生物信息学可以从中提取有效信息,为前列腺癌分子机制研究提供思路及数据基础。
[Abstract]:Objective to analyze the gene expression microarray of prostate cancer by bioinformatics, and to study the function and regulatory network of differentially expressed genes in prostate cancer. Methods the microarray data of prostate cancer gene expression were obtained from GEO database. The data mining and bioinformatics analysis were carried out by using R software and R software package, such as affyrium limma pheatmapggplot2. The differential expression genes and their regulatory networks were analyzed by using the bioinformatics tool DAVIDGene MANIA. The results showed that prostate cancer and adjacent cancer groups were selected together. There are 56 differentially expressed genes. There were 15 up-regulated genes and 41 down-regulated genes in prostate cancer and paracancerous tissues. Among them, 9 genes, such as cav1sslc16a2cav2slc16a5, magi2ptrfptrfptrftol mod 1abcc6, were enriched into the "cell membrane subgroup" subsets under the classification of "cell function". Cav1Cav2Ptrf may play an important role in the pathogenesis and development of prostate cancer. Conclusion there is a complex regulatory network between the differentially expressed genes in prostate cancer and paracancerous tissues. Bioinformatics can extract effective information from it and provide ideas and data basis for the study of molecular mechanism of prostate cancer.
【作者单位】：安徽医科大学深圳临床学院(深圳市第二人民医院);深圳市第二人民医院泌尿外科;深圳大学附属罗湖医院泌尿外科;
【基金】：国家重点基础研究发展计划(973计划)项目(编号:2014CB745200) 国家自然科学基金(编号:81301740)
【分类号】：R737.25

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