MicroRNA-150-5p对肝细胞癌细胞增殖、凋亡和周期的影响

发布时间：2018-03-03 04:04

本文选题：MicroRNA--p　切入点：增殖　出处：《中国现代医学杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的研究microRNA-150-5p(miR-150-5p)在人正常肝细胞和肝细胞癌细胞中的表达,及其对肝细胞癌细胞增殖、凋亡和周期的影响及潜在机制。方法通过实时荧光定量聚合酶链反应(qRT-PCR)检测各处理组细胞中的miR-150-5p的表达水平;MTT法检测miR-150-5p对肝细胞癌细胞增殖的影响;流式细胞术检测miR-150-5p对肝细胞癌细胞凋亡和周期的影响;荧光素酶报告基因实验检测锌指蛋白609是否为miR-150-5p的直接靶基因。结果 miR-150-5p在肝细胞癌细胞系中的表达较人正常肝细胞细胞系降低,miR-150-5p可抑制肝细胞癌细胞的增殖,促进肝细胞癌细胞的凋亡,并阻滞细胞周期于G_1期。miR-150-5p在肝细胞癌中发挥肿瘤抑制分子作用的潜在机制为直接靶向抑制ZNF609的表达。结论 miR-150-5p在肝细胞癌细胞中表达降低,并可通过调控ZNF609的表达而调控肝细胞癌细胞增殖、凋亡和周期。
[Abstract]:Objective to study the expression of microRNA-150-5pmmiR-150-5p in human normal hepatocytes and hepatocellular carcinoma cells, and to investigate the effect of microRNA-150-5pmmiR-150-5p on the proliferation of hepatocellular carcinoma cells. Methods the effect of miR-150-5p on the proliferation of hepatocellular carcinoma cells was detected by real-time fluorescence quantitative polymerase chain reaction (QRT-PCR). The effect of miR-150-5p on apoptosis and cell cycle of hepatocellular carcinoma was detected by flow cytometry. Luciferase reporter gene assay was used to detect whether zinc finger protein 609 was a direct target gene of miR-150-5p. Results the expression of miR-150-5p in hepatocellular carcinoma cell line was lower than that in human normal hepatoma cell line. The potential mechanism of promoting the apoptosis of hepatocellular carcinoma cells and arresting the cell cycle in G1 phase. MiR-150-5p plays a role in tumor suppressor molecules in hepatocellular carcinoma is the direct targeting inhibition of ZNF609 expression. Conclusion the expression of miR-150-5p in hepatocellular carcinoma cells is decreased. The proliferation, apoptosis and cycle of hepatocellular carcinoma cells can be regulated by regulating the expression of ZNF609.
【作者单位】：江苏联合职业技术学院南京卫生分院;江苏省人民医院肝脏外科;
【分类号】：R735.7

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